Laser activated fluorescence measurements and morphological features: An in vivo study of clearance time of fluorescein isothiocyanate tagged cell markers

I. Gannot, G. Gannot, A. Garashi, A. Gandjbakhche, A. Buchner, Y. Keisari

Research output: Contribution to journalArticlepeer-review

Abstract

Fourteen BALB/c mice were divided into two groups. One group served as the control and the second group was injected with a squamous cell carcinoma cell line to the tongue. After tumor development (1-4 weeks), mice were injected with a FITC conjugated CD3 marker to their tongues. Immediately after the marker injection, the clearance of the marker was measured using a laser spectroscopy system. The markers were excited by an argon laser at 488 nm and the fluorescence signal was measured as a function of time. A biopsy was taken from every mouse after the procedure and the excised tissue was histologically evaluated. Analysis of clearance times revealed a second order exponential decay for both groups with a slower pace of signal clearance for the sick mice.

Original languageEnglish (US)
Pages (from-to)14-19
Number of pages6
JournalJournal of biomedical optics
Volume7
Issue number1
DOIs
StatePublished - Jan 2002
Externally publishedYes

Keywords

  • Fluorescence
  • Lasers
  • Tissues

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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