Large-scale purification of inactivated hepatitis A virus by centrifugation in non-ionic gradients

D. R. Dubois, K. H. Eckels, J. Ticehurst, L. N. Binn, R. L. Timchak, D. A. Barvir, C. T. Rankin, S. P. O'Neill

Research output: Contribution to journalArticlepeer-review

Abstract

Formalin-inactivated hepatitis A virus (HAV) can be purified for vaccine preparation by centrifugation in Renografin-76 (diatrizoate meglumine and diatrizoate sodium) gradients. Both continuous-flow rate-zonal and isopycnic methods were used for the separation of a major antigen component from minor antigen and host protein. The major antigen component, which appeared to contain complete virions by electron microscopy, could be recovered from gradients and accounted for approximately one third of the total antigen in the starting material. The HAV-specific purified antigen could be enriched 200-300-fold by either centrifugation procedure. The purified HAV antigen, when adsorbed to alum and inoculated into mice, was found to be highly immunogenic.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalJournal of Virological Methods
Volume32
Issue number2-3
DOIs
StatePublished - May 1991
Externally publishedYes

Keywords

  • Centrifugation
  • Hepatitis A vaccine
  • Hepatitis A virus
  • Purification

ASJC Scopus subject areas

  • Virology

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