Large-scale N-glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions

Xiaoqiang Qiao, Dingyin Tao, Yanyan Qu, Liangliang Sun, Liang Gao, Xiaodan Zhang, Zhen Liang, Lihua Zhang, Yukui Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 nonredundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.

Original languageEnglish (US)
Pages (from-to)4274-4278
Number of pages5
JournalProteomics
Volume11
Issue number21
DOIs
StatePublished - Jan 1 2011

Keywords

  • Glycoproteomics
  • Hydrazide chemistry
  • Insoluble and soluble protein fraction
  • Ionic liquid
  • N-glycoproteome
  • Rat brain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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