Large-Scale Measurement of Absolute Protein Glycosylation Stoichiometry

Shisheng Sun, Hui Zhang

Research output: Contribution to journalArticle

Abstract

Protein glycosylation is one of the most important protein modifications. Glycosylation site occupancy alteration has been implicated in human diseases and cancers. However, current glycoproteomic methods focus on the identification and quantification of glycosylated peptides and glycosylation sites but not glycosylation occupancy or glycoform stoichiometry. Here we describe a method for large-scale determination of the absolute glycosylation stoichiometry using three independent relative ratios. Using this method, we determined 117 absolute N-glycosylation occupancies in OVCAR-3 cells. Finally, we investigated the possible functions and the determinants for partial glycosylation. (Graph Presented).

Original languageEnglish (US)
Pages (from-to)6479-6482
Number of pages4
JournalAnalytical chemistry
Volume87
Issue number13
DOIs
StatePublished - Jul 7 2015

ASJC Scopus subject areas

  • Analytical Chemistry

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