Large-scale exploration of gene-gene interactions in prostate cancer using a multistage genome-wide association study

Julia Ciampa, Meredith Yeager, Laufey Amundadottir, Kevin Jacobs, Peter Kraft, Charles Chung, Sholom Wacholder, Kai Yu, William Wheeler, Michael J. Thun, W. Ryan Divers, Susan Gapstur, Demetrius Albanes, Jarmo Virtamo, Stephanie Weinstein, Edward Giovannucci, Walter C. Willett, Geraldine Cancel-Tassin, Olivier Cussenot, Antoine ValeriDavid Hunter, Robert Hoover, Gilles Thomas, Stephen Chanock, Nilanjan Chatterjee

Research output: Contribution to journalArticle

Abstract

Recent genome-wide association studies have identified independent susceptibility loci for prostate cancer that could influence risk through interaction with other, possibly undetected, susceptibility loci. We explored evidence of interaction between pairs of 13 known susceptibility loci and single nucleotide polymorphisms (SNP) across the genome to generate hypotheses about the functionality of prostate cancer susceptibility regions. We used data from Cancer Genetic Markers of Susceptibility: Stage I included 523,841 SNPs in 1,175 cases and 1,100 controls; Stage II included 27,383 SNPs in an additional 3,941 cases and 3,964 controls. Power calculations assessed the magnitude of interactions our study is likely to detect. Logistic regression was used with alternative methods that exploit constraints of gene-gene independence between unlinked loci to increase power. Our empirical evaluation demonstrated that an empirical Bayes (EB) technique is powerful and robust to possible violation of the independence assumption. Our EB analysis identified several noteworthy interacting SNP pairs, although none reached genome-wide significance. We highlight a Stage II interaction between the major prostate cancer susceptibility locus in the subregion of 8q24 that contains POU5F1B and an intronic SNP in the transcription factor EPAS1, which has potentially important functional implications for 8q24. Another noteworthy result involves interaction of a known prostate cancer susceptibility marker near the prostate protease genes KLK2 and KLK3 with an intronic SNP in PRXX2. Overall, the interactions we have identified merit follow-up study, particularly the EPAS1 interaction, which has implications not only in prostate cancer but also in other epithelial cancers that are associated with the 8q24 locus.

Original languageEnglish (US)
Pages (from-to)3287-3295
Number of pages9
JournalCancer Research
Volume71
Issue number9
DOIs
StatePublished - May 1 2011
Externally publishedYes

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Genome-Wide Association Study
Single Nucleotide Polymorphism
Prostatic Neoplasms
Genes
Genome
Genetic Predisposition to Disease
Genetic Markers
Prostate
Neoplasms
Peptide Hydrolases
Transcription Factors
Logistic Models

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Large-scale exploration of gene-gene interactions in prostate cancer using a multistage genome-wide association study. / Ciampa, Julia; Yeager, Meredith; Amundadottir, Laufey; Jacobs, Kevin; Kraft, Peter; Chung, Charles; Wacholder, Sholom; Yu, Kai; Wheeler, William; Thun, Michael J.; Divers, W. Ryan; Gapstur, Susan; Albanes, Demetrius; Virtamo, Jarmo; Weinstein, Stephanie; Giovannucci, Edward; Willett, Walter C.; Cancel-Tassin, Geraldine; Cussenot, Olivier; Valeri, Antoine; Hunter, David; Hoover, Robert; Thomas, Gilles; Chanock, Stephen; Chatterjee, Nilanjan.

In: Cancer Research, Vol. 71, No. 9, 01.05.2011, p. 3287-3295.

Research output: Contribution to journalArticle

Ciampa, J, Yeager, M, Amundadottir, L, Jacobs, K, Kraft, P, Chung, C, Wacholder, S, Yu, K, Wheeler, W, Thun, MJ, Divers, WR, Gapstur, S, Albanes, D, Virtamo, J, Weinstein, S, Giovannucci, E, Willett, WC, Cancel-Tassin, G, Cussenot, O, Valeri, A, Hunter, D, Hoover, R, Thomas, G, Chanock, S & Chatterjee, N 2011, 'Large-scale exploration of gene-gene interactions in prostate cancer using a multistage genome-wide association study', Cancer Research, vol. 71, no. 9, pp. 3287-3295. https://doi.org/10.1158/0008-5472.CAN-10-2646
Ciampa, Julia ; Yeager, Meredith ; Amundadottir, Laufey ; Jacobs, Kevin ; Kraft, Peter ; Chung, Charles ; Wacholder, Sholom ; Yu, Kai ; Wheeler, William ; Thun, Michael J. ; Divers, W. Ryan ; Gapstur, Susan ; Albanes, Demetrius ; Virtamo, Jarmo ; Weinstein, Stephanie ; Giovannucci, Edward ; Willett, Walter C. ; Cancel-Tassin, Geraldine ; Cussenot, Olivier ; Valeri, Antoine ; Hunter, David ; Hoover, Robert ; Thomas, Gilles ; Chanock, Stephen ; Chatterjee, Nilanjan. / Large-scale exploration of gene-gene interactions in prostate cancer using a multistage genome-wide association study. In: Cancer Research. 2011 ; Vol. 71, No. 9. pp. 3287-3295.
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AU - Kraft, Peter

AU - Chung, Charles

AU - Wacholder, Sholom

AU - Yu, Kai

AU - Wheeler, William

AU - Thun, Michael J.

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AU - Gapstur, Susan

AU - Albanes, Demetrius

AU - Virtamo, Jarmo

AU - Weinstein, Stephanie

AU - Giovannucci, Edward

AU - Willett, Walter C.

AU - Cancel-Tassin, Geraldine

AU - Cussenot, Olivier

AU - Valeri, Antoine

AU - Hunter, David

AU - Hoover, Robert

AU - Thomas, Gilles

AU - Chanock, Stephen

AU - Chatterjee, Nilanjan

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