Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens

Nuntra Suwantarat, Justin B. Dalton, Richard Lee, Rachel Green, Warda Memon, Karen C Carroll, Stefan Riedel, Sean Xiang Zhang

Research output: Contribution to journalArticle

Abstract

We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.

Original languageEnglish (US)
Pages (from-to)54-56
Number of pages3
JournalDiagnostic Microbiology and Infectious Disease
Volume82
Issue number1
DOIs
StatePublished - May 1 2015

Fingerprint

Immunoassay
Cerebrospinal Fluid
Antigens
Serum
Cryptococcosis
Immunoenzyme Techniques
Patient Care
Sensitivity and Specificity

Keywords

  • Antigen
  • Cerebrospinal fluid
  • Cryptococcus
  • Lateral flow immunoassay
  • Serum

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Medicine(all)

Cite this

Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens. / Suwantarat, Nuntra; Dalton, Justin B.; Lee, Richard; Green, Rachel; Memon, Warda; Carroll, Karen C; Riedel, Stefan; Zhang, Sean Xiang.

In: Diagnostic Microbiology and Infectious Disease, Vol. 82, No. 1, 01.05.2015, p. 54-56.

Research output: Contribution to journalArticle

@article{bad73d3861244f0ba87ea84e4f1763fe,
title = "Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens",
abstract = "We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97{\%} concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100{\%} and specificity of 99.6{\%} for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.",
keywords = "Antigen, Cerebrospinal fluid, Cryptococcus, Lateral flow immunoassay, Serum",
author = "Nuntra Suwantarat and Dalton, {Justin B.} and Richard Lee and Rachel Green and Warda Memon and Carroll, {Karen C} and Stefan Riedel and Zhang, {Sean Xiang}",
year = "2015",
month = "5",
day = "1",
doi = "10.1016/j.diagmicrobio.2015.01.012",
language = "English (US)",
volume = "82",
pages = "54--56",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens

AU - Suwantarat, Nuntra

AU - Dalton, Justin B.

AU - Lee, Richard

AU - Green, Rachel

AU - Memon, Warda

AU - Carroll, Karen C

AU - Riedel, Stefan

AU - Zhang, Sean Xiang

PY - 2015/5/1

Y1 - 2015/5/1

N2 - We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.

AB - We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.

KW - Antigen

KW - Cerebrospinal fluid

KW - Cryptococcus

KW - Lateral flow immunoassay

KW - Serum

UR - http://www.scopus.com/inward/record.url?scp=84928375639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928375639&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2015.01.012

DO - 10.1016/j.diagmicrobio.2015.01.012

M3 - Article

C2 - 25698631

AN - SCOPUS:84928375639

VL - 82

SP - 54

EP - 56

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 1

ER -