Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse development

Minoru S.H. Ko, John R. Kitchen, Xiaohong Wang, Tracy A. Threat, Xueqian Wang, Aki Hasegawa, Tong Sun, Marija J. Grahovac, George J. Kargul, Meng K. Lim, Yu Shun Cui, Yuri Sano, Tetsuya Tanaka, Yuling Liang, Scott Mason, Paul D. Paonessa, Althea D. Sauls, Grace E. DePalma, Rana Sharara, Lucy B. RoweJanan Eppig, Chris Morrell, Hirofumi Doi

Research output: Contribution to journalArticle

Abstract

Little is known about gene action in the preimplantation events that initiate mammalian development. Based on cDNA collections made from each stage from egg to blastocyst, 25438 3'-ESTs were derived, and represent 9718 genes, half of them novel. Thus, a considerable fraction of mammalian genes is dedicated to embryonic expression. This study reveals profound changes in gene expression that include the transient induction of transcripts at each stage. These results raise the possibility that development is driven by the action of a series of stage-specific expressed genes. The new genes, 798 of them placed on the mouse genetic map, provide entry points for analyses of human and mouse developmental disorders.

Original languageEnglish (US)
Pages (from-to)1737-1749
Number of pages13
JournalDevelopment
Volume127
Issue number8
StatePublished - Apr 2000

Keywords

  • EST
  • Gene mapping
  • Mouse development
  • Preimplantation
  • Stage-specific gene
  • cDNA analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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  • Cite this

    Ko, M. S. H., Kitchen, J. R., Wang, X., Threat, T. A., Wang, X., Hasegawa, A., Sun, T., Grahovac, M. J., Kargul, G. J., Lim, M. K., Cui, Y. S., Sano, Y., Tanaka, T., Liang, Y., Mason, S., Paonessa, P. D., Sauls, A. D., DePalma, G. E., Sharara, R., ... Doi, H. (2000). Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse development. Development, 127(8), 1737-1749.