Laparoscopic embryo transfer in domestic sheep: A preliminary study

M. C. Schiewe, M. Bush, L. S. Stuart, D. E. Wildt

Research output: Contribution to journalArticle

Abstract

An effective, minor-invasive technique for embryo transfer in sheep was developed using a laparoscopic transabdominal approach. Twelve recipient ewes received embryos either by conventional laparotomy or by laparoscopy. The estrous cycle of recipient ewes was synchronized using a progestagen-impregnated vaginal pessary/pregnant mares' serum gonadotropin treatment regimen. Donor ewes were superovulated with follicle stimulating hormone or human menopausal gonadotropin, bred with a ram of one breed and laparoscopically inseminated in utero with semen from a different sheep breed. Five to six days after estrus, embryos were transferred laparoscopically into the terminal one-half of the recipient's uterine horn ipsilateral to the ovary with prominent corpus luteum development. Pregnancy was diagnosed by transrectal ultrasonic procedures, and by direct laparoscopic examination of the uterus. Of six laparoscopic transfers, three resulted in single births; one of six laparotomy transfers resulted in a live birth. Breed appearances of the four lambs born indicated that two of the offspring resulted from laparoscopic artificial insemination of the donor ewe. The results demonstrated that laparoscopic transfer of embryos was a rapid and safe procedure, easily applied to an ovine embryo transfer program and with potential for similiar studies in other species.

Original languageEnglish (US)
Pages (from-to)675-682
Number of pages8
JournalTheriogenology
Volume22
Issue number6
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Domestic Sheep
Embryo Transfer
embryo transfer
ewes
Sheep
sheep
Laparotomy
laparotomy
Embryonic Structures
breeds
Pessaries
Equine Gonadotropins
Menotropins
embryo (animal)
Estrous Cycle
Corpus Luteum
Estrus
Live Birth
Follicle Stimulating Hormone
Progestins

Keywords

  • Embryo Transfer
  • Laparoscopy
  • Sheep

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Schiewe, M. C., Bush, M., Stuart, L. S., & Wildt, D. E. (1984). Laparoscopic embryo transfer in domestic sheep: A preliminary study. Theriogenology, 22(6), 675-682. https://doi.org/10.1016/0093-691X(84)90497-7

Laparoscopic embryo transfer in domestic sheep : A preliminary study. / Schiewe, M. C.; Bush, M.; Stuart, L. S.; Wildt, D. E.

In: Theriogenology, Vol. 22, No. 6, 1984, p. 675-682.

Research output: Contribution to journalArticle

Schiewe, MC, Bush, M, Stuart, LS & Wildt, DE 1984, 'Laparoscopic embryo transfer in domestic sheep: A preliminary study', Theriogenology, vol. 22, no. 6, pp. 675-682. https://doi.org/10.1016/0093-691X(84)90497-7
Schiewe, M. C. ; Bush, M. ; Stuart, L. S. ; Wildt, D. E. / Laparoscopic embryo transfer in domestic sheep : A preliminary study. In: Theriogenology. 1984 ; Vol. 22, No. 6. pp. 675-682.
@article{d665e10842cc4f3cb5398ecea9b87fa6,
title = "Laparoscopic embryo transfer in domestic sheep: A preliminary study",
abstract = "An effective, minor-invasive technique for embryo transfer in sheep was developed using a laparoscopic transabdominal approach. Twelve recipient ewes received embryos either by conventional laparotomy or by laparoscopy. The estrous cycle of recipient ewes was synchronized using a progestagen-impregnated vaginal pessary/pregnant mares' serum gonadotropin treatment regimen. Donor ewes were superovulated with follicle stimulating hormone or human menopausal gonadotropin, bred with a ram of one breed and laparoscopically inseminated in utero with semen from a different sheep breed. Five to six days after estrus, embryos were transferred laparoscopically into the terminal one-half of the recipient's uterine horn ipsilateral to the ovary with prominent corpus luteum development. Pregnancy was diagnosed by transrectal ultrasonic procedures, and by direct laparoscopic examination of the uterus. Of six laparoscopic transfers, three resulted in single births; one of six laparotomy transfers resulted in a live birth. Breed appearances of the four lambs born indicated that two of the offspring resulted from laparoscopic artificial insemination of the donor ewe. The results demonstrated that laparoscopic transfer of embryos was a rapid and safe procedure, easily applied to an ovine embryo transfer program and with potential for similiar studies in other species.",
keywords = "Embryo Transfer, Laparoscopy, Sheep",
author = "Schiewe, {M. C.} and M. Bush and Stuart, {L. S.} and Wildt, {D. E.}",
year = "1984",
doi = "10.1016/0093-691X(84)90497-7",
language = "English (US)",
volume = "22",
pages = "675--682",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Laparoscopic embryo transfer in domestic sheep

T2 - A preliminary study

AU - Schiewe, M. C.

AU - Bush, M.

AU - Stuart, L. S.

AU - Wildt, D. E.

PY - 1984

Y1 - 1984

N2 - An effective, minor-invasive technique for embryo transfer in sheep was developed using a laparoscopic transabdominal approach. Twelve recipient ewes received embryos either by conventional laparotomy or by laparoscopy. The estrous cycle of recipient ewes was synchronized using a progestagen-impregnated vaginal pessary/pregnant mares' serum gonadotropin treatment regimen. Donor ewes were superovulated with follicle stimulating hormone or human menopausal gonadotropin, bred with a ram of one breed and laparoscopically inseminated in utero with semen from a different sheep breed. Five to six days after estrus, embryos were transferred laparoscopically into the terminal one-half of the recipient's uterine horn ipsilateral to the ovary with prominent corpus luteum development. Pregnancy was diagnosed by transrectal ultrasonic procedures, and by direct laparoscopic examination of the uterus. Of six laparoscopic transfers, three resulted in single births; one of six laparotomy transfers resulted in a live birth. Breed appearances of the four lambs born indicated that two of the offspring resulted from laparoscopic artificial insemination of the donor ewe. The results demonstrated that laparoscopic transfer of embryos was a rapid and safe procedure, easily applied to an ovine embryo transfer program and with potential for similiar studies in other species.

AB - An effective, minor-invasive technique for embryo transfer in sheep was developed using a laparoscopic transabdominal approach. Twelve recipient ewes received embryos either by conventional laparotomy or by laparoscopy. The estrous cycle of recipient ewes was synchronized using a progestagen-impregnated vaginal pessary/pregnant mares' serum gonadotropin treatment regimen. Donor ewes were superovulated with follicle stimulating hormone or human menopausal gonadotropin, bred with a ram of one breed and laparoscopically inseminated in utero with semen from a different sheep breed. Five to six days after estrus, embryos were transferred laparoscopically into the terminal one-half of the recipient's uterine horn ipsilateral to the ovary with prominent corpus luteum development. Pregnancy was diagnosed by transrectal ultrasonic procedures, and by direct laparoscopic examination of the uterus. Of six laparoscopic transfers, three resulted in single births; one of six laparotomy transfers resulted in a live birth. Breed appearances of the four lambs born indicated that two of the offspring resulted from laparoscopic artificial insemination of the donor ewe. The results demonstrated that laparoscopic transfer of embryos was a rapid and safe procedure, easily applied to an ovine embryo transfer program and with potential for similiar studies in other species.

KW - Embryo Transfer

KW - Laparoscopy

KW - Sheep

UR - http://www.scopus.com/inward/record.url?scp=0039313946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0039313946&partnerID=8YFLogxK

U2 - 10.1016/0093-691X(84)90497-7

DO - 10.1016/0093-691X(84)90497-7

M3 - Article

AN - SCOPUS:0039313946

VL - 22

SP - 675

EP - 682

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 6

ER -