Laparoscopic embryo transfer in domestic sheep: A preliminary study

M. C. Schiewe, M. Bush, L. S. Stuart, D. E. Wildt

Research output: Contribution to journalArticle

Abstract

An effective, minor-invasive technique for embryo transfer in sheep was developed using a laparoscopic transabdominal approach. Twelve recipient ewes received embryos either by conventional laparotomy or by laparoscopy. The estrous cycle of recipient ewes was synchronized using a progestagen-impregnated vaginal pessary/pregnant mares' serum gonadotropin treatment regimen. Donor ewes were superovulated with follicle stimulating hormone or human menopausal gonadotropin, bred with a ram of one breed and laparoscopically inseminated in utero with semen from a different sheep breed. Five to six days after estrus, embryos were transferred laparoscopically into the terminal one-half of the recipient's uterine horn ipsilateral to the ovary with prominent corpus luteum development. Pregnancy was diagnosed by transrectal ultrasonic procedures, and by direct laparoscopic examination of the uterus. Of six laparoscopic transfers, three resulted in single births; one of six laparotomy transfers resulted in a live birth. Breed appearances of the four lambs born indicated that two of the offspring resulted from laparoscopic artificial insemination of the donor ewe. The results demonstrated that laparoscopic transfer of embryos was a rapid and safe procedure, easily applied to an ovine embryo transfer program and with potential for similiar studies in other species.

Original languageEnglish (US)
Pages (from-to)675-682
Number of pages8
JournalTheriogenology
Volume22
Issue number6
DOIs
StatePublished - Dec 1984

Keywords

  • Embryo Transfer
  • Laparoscopy
  • Sheep

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

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    Schiewe, M. C., Bush, M., Stuart, L. S., & Wildt, D. E. (1984). Laparoscopic embryo transfer in domestic sheep: A preliminary study. Theriogenology, 22(6), 675-682. https://doi.org/10.1016/0093-691X(84)90497-7