Lamin-dependent localization of UNC-84, a protein required for nuclear migration in Caenorhabditis elegans

Kenneth K. Lee, Daniel Starr, Merav Cohen, Jun Liu, Min Han, Katherine Lee Wilson, Yosef Gruenbaum

Research output: Contribution to journalArticle

Abstract

Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear periphery until late anaphase, similar to known inner nuclear membrane proteins. UNC-84 protein is first detected at the 26-cell stage and thereafter is present in most cells during development and in adults. UNC-84 is properly expressed in unc-83 and anc-1 lines, which have phenotypes similar to unc-84, suggesting that neither the expression nor nuclear envelope localization of UNC-84 depends on UNC-83 or ANC-1 proteins. The envelope localization of Ce-lamin, Ce-emerin, Ce-MAN1, and nucleoporins are unaffected by the loss of UNC-84. UNC-84 is not required for centrosome attachment to the nucleus because centrosomes are localized normally in unc-84 hyp7 cells despite a nuclear migration defect. Models for UNC-84 localization are discussed.

Original languageEnglish (US)
Pages (from-to)892-901
Number of pages10
JournalMolecular Biology of the Cell
Volume13
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

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Lamins
Nuclear Envelope
Caenorhabditis elegans
Nuclear Proteins
Centrosome
Nuclear Pore Complex Proteins
Anaphase
Proteins
Mitosis
Membrane Proteins
Phenotype
Mutation
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Lamin-dependent localization of UNC-84, a protein required for nuclear migration in Caenorhabditis elegans. / Lee, Kenneth K.; Starr, Daniel; Cohen, Merav; Liu, Jun; Han, Min; Wilson, Katherine Lee; Gruenbaum, Yosef.

In: Molecular Biology of the Cell, Vol. 13, No. 3, 2002, p. 892-901.

Research output: Contribution to journalArticle

Lee, Kenneth K. ; Starr, Daniel ; Cohen, Merav ; Liu, Jun ; Han, Min ; Wilson, Katherine Lee ; Gruenbaum, Yosef. / Lamin-dependent localization of UNC-84, a protein required for nuclear migration in Caenorhabditis elegans. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 3. pp. 892-901.
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