TY - JOUR
T1 - Lactosylceramide mediates tumor necrosis factor-α-induced intercellular adhesion molecule-1 (ICAM-1) expression and the adhesion of neutrophil in human umbilical vein endothelial cells
AU - Bhunia, Anil Kumar
AU - Arai, Toshiyuki
AU - Bulkley, Gregory
AU - Chatterjee, Subroto
PY - 1998/12/18
Y1 - 1998/12/18
N2 - The endothelial expression of adhesion molecules by proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) has been suggested to contribute to the initiation of atherosclerotic plaque formation. Since lactosylceramide (LacCer) accumulates in large quantities in human atherosclerotic plaque, we have explored its role in TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells and their consequent adhesion to polymorphonuclear leukocytes (PMNs). We found that TNF-α increased LacCer synthesis by way of stimulating the activity of UDP-galactose:glucosylceramide β(1→4)galactosyltransferase in a time-dependent fashion. The TNF-α- induced expression of ICAM-1 was abrogated by D-1-phenyl-2-decanoylamino-3- morpholino-1-propanol (D-PDMP), an inhibitor of UDP- galactose:glucosylceramide/β(1→4)-galactosyltransferase. However, the addition of LacCer reversed the D-PDMP effect on TNF-α-induced ICAM-I expression in human umbilical vein endothelial cells. Northern hybridization analysis of mRNA levels and enzyme-linked immunosorbent assays revealed that LacCer (5μM) specifically stimulated ICAM-1 at both the transcriptional and translational levels. This was accompanied by the adhesion of PMNs, which was visualized by confocal microscopy. Further studies revealed that LacCer stimulated the endogenous generation of superoxide radicals (O2/·-) about 5-fold compared with the control by specifically activating plasma membrane- associated NADPH-dependent oxidase. This phenomenon was blocked by the antioxidant N-acetyl-L-cysteine, pyrrolidine dithiocarbamate, and the NADPH oxidase inhibitor, diphenylene iodonium. Overexpression of endogeneous CuZn- superoxide dismutase via an adenoviral vector carrying cDNA for CuZn- superoxide dismutase, also inhibited LacCer-induced ICAM-1 expression in endothelial cells. In sum, our findings suggest that LacCer may play the role of a lipid second messenger in TNF-α-induced pathogenesis by activating an oxidant-sensitive transcriptional pathway that leads to the adhesion of PMNs to endothelial cells.
AB - The endothelial expression of adhesion molecules by proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) has been suggested to contribute to the initiation of atherosclerotic plaque formation. Since lactosylceramide (LacCer) accumulates in large quantities in human atherosclerotic plaque, we have explored its role in TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells and their consequent adhesion to polymorphonuclear leukocytes (PMNs). We found that TNF-α increased LacCer synthesis by way of stimulating the activity of UDP-galactose:glucosylceramide β(1→4)galactosyltransferase in a time-dependent fashion. The TNF-α- induced expression of ICAM-1 was abrogated by D-1-phenyl-2-decanoylamino-3- morpholino-1-propanol (D-PDMP), an inhibitor of UDP- galactose:glucosylceramide/β(1→4)-galactosyltransferase. However, the addition of LacCer reversed the D-PDMP effect on TNF-α-induced ICAM-I expression in human umbilical vein endothelial cells. Northern hybridization analysis of mRNA levels and enzyme-linked immunosorbent assays revealed that LacCer (5μM) specifically stimulated ICAM-1 at both the transcriptional and translational levels. This was accompanied by the adhesion of PMNs, which was visualized by confocal microscopy. Further studies revealed that LacCer stimulated the endogenous generation of superoxide radicals (O2/·-) about 5-fold compared with the control by specifically activating plasma membrane- associated NADPH-dependent oxidase. This phenomenon was blocked by the antioxidant N-acetyl-L-cysteine, pyrrolidine dithiocarbamate, and the NADPH oxidase inhibitor, diphenylene iodonium. Overexpression of endogeneous CuZn- superoxide dismutase via an adenoviral vector carrying cDNA for CuZn- superoxide dismutase, also inhibited LacCer-induced ICAM-1 expression in endothelial cells. In sum, our findings suggest that LacCer may play the role of a lipid second messenger in TNF-α-induced pathogenesis by activating an oxidant-sensitive transcriptional pathway that leads to the adhesion of PMNs to endothelial cells.
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U2 - 10.1074/jbc.273.51.34349
DO - 10.1074/jbc.273.51.34349
M3 - Article
C2 - 9852101
AN - SCOPUS:0032545259
SN - 0021-9258
VL - 273
SP - 34349
EP - 34357
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 51
ER -