Lactobacillus acidophilus attenuates downregulation of DRA function and expression in inflammatory models

Varsha Singh, Anoop Kumar, Geetu Raheja, Arivarasu N. Anbazhagan, Shubha Priyamvada, Seema Saksena, Muhammad Nauman Jhandier, Ravinder K. Gill, Waddah A. Alrefai, Alip Borthakur, Pradeep K. Dudeja

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Probiotics, including Lactobacilli, are commensal bacteria that have been used in clinical trials and experimental models for the prevention and treatment of diarrheal disorders. Our previous studies have shown that Lactobacillus acidophilus (LA) and its culture supernatant (CS) stimulated Cl‾/HCO‾3 exchange activity, acutely via an increase in the surface levels of downregulated in adenoma (DRA, SLC26A3) and in long-term treatments via increasing its expression involving transcriptional mechanisms. However, the role of LA in modulating DRA activity under inflammatory conditions is not known. Current in vitro studies using human intestinal epithelial Caco-2 cells examined the efficacy of LA or its CS in counteracting the inhibitory effects of interferon-")/ (IFN-γ) on Cl‾/HCO‾3exchange activity. Pretreatment of cells with LA or LA-CS for 1 h followed by coincubation with IFN-γ significantly alleviated the inhibitory effects of IFN-γ on Cl‾/HCO‾3 exchange activity. In the in vivo model of dextran sulfate sodium-induced experimental colitis (3% in drinking water for 7 days) in C57BL/6J mice, administration of live LA (3 × 109 colony-forming units) via oral gavage attenuated colonic inflammation. LA administration also counteracted the colitis-induced decrease in DRA mRNA and protein levels. Efficacy of LA or its secreted soluble factors in alleviating inflammation and inflammation-associated dysregulation of DRA activity could justify their therapeutic potential in inflammatory diarrheal diseases.

Original languageEnglish (US)
Pages (from-to)G623-G631
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number6
StatePublished - Sep 15 2014
Externally publishedYes


  • Caco-2
  • Dextran sulfate sodium
  • Diarrhea
  • Downregulated in adenoma
  • Interferon-γ
  • Probiotics

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)


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