Lactacystin inhibits 3T3-L1 adipocyte differentiation through induction of CHOP-10 expression

Xi Li; Hai Yan Huang; Jie Gen Chen; Lin Jiang; Hong Lei Liu; De Guo Liu; Tan Jing Song; Qun He; Chun Gu Ma; Duan Ma; Hou Yan Song; Qi Qun Tang

doi:10.1016/j.bbrc.2006.08.188

Lactacystin inhibits 3T3-L1 adipocyte differentiation through induction of CHOP-10 expression

Xi Li, Hai Yan Huang, Jie Gen Chen, Lin Jiang, Hong Lei Liu, De Guo Liu, Tan Jing Song, Qun He, Chun Gu Ma, Duan Ma, Hou Yan Song, Qi Qun Tang

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Hormonal induction triggers a cascade leading to the expression of CCAAT/enhancer-binding protein(C/EBP)α and peroxisome proliferator-activated receptor (PPAR) γ, C/EBPα, and PPARγ turns on series of adipocyte genes that give rise to the adipocyte phenotype. Previous findings indicate that C/EBPβ, a transcriptional activator of the C/EBPα and PPARγ genes, is rapidly expressed after induction, but lacks DNA-binding activity and therefore cannot activate transcription of the C/EBPα and PPARγ genes early in the differentiation program. Acquisition of DNA-binding activity of C/EBPβ occurs when CHOP-10, a dominant-negative form of C/EBP family members, is down-regulated and becomes hyperphosphorylated as preadipocytes traverse the G₁-S checkpoint of mitotic clonal expansion. Evidences are presented in this report that lactacystin, a proteasome inhibitor, up-regulated the CHOP-10 expression, blocked the DNA-binding activity of C/EBPβ, and subsequently inhibited MCE as well as adipocyte differentiation.

Original language	English (US)
Pages (from-to)	1-6
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	350
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.08.188
State	Published - Nov 10 2006
Externally published	Yes

Keywords

C/EBPα
C/EBPβ
DNA-binding activity
Mitotic clonal expansion
PPARγ

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/j.bbrc.2006.08.188

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title = "Lactacystin inhibits 3T3-L1 adipocyte differentiation through induction of CHOP-10 expression",

abstract = "Hormonal induction triggers a cascade leading to the expression of CCAAT/enhancer-binding protein(C/EBP)α and peroxisome proliferator-activated receptor (PPAR) γ, C/EBPα, and PPARγ turns on series of adipocyte genes that give rise to the adipocyte phenotype. Previous findings indicate that C/EBPβ, a transcriptional activator of the C/EBPα and PPARγ genes, is rapidly expressed after induction, but lacks DNA-binding activity and therefore cannot activate transcription of the C/EBPα and PPARγ genes early in the differentiation program. Acquisition of DNA-binding activity of C/EBPβ occurs when CHOP-10, a dominant-negative form of C/EBP family members, is down-regulated and becomes hyperphosphorylated as preadipocytes traverse the G1-S checkpoint of mitotic clonal expansion. Evidences are presented in this report that lactacystin, a proteasome inhibitor, up-regulated the CHOP-10 expression, blocked the DNA-binding activity of C/EBPβ, and subsequently inhibited MCE as well as adipocyte differentiation.",

keywords = "C/EBPα, C/EBPβ, DNA-binding activity, Mitotic clonal expansion, PPARγ",

author = "Xi Li and Huang, {Hai Yan} and Chen, {Jie Gen} and Lin Jiang and Liu, {Hong Lei} and Liu, {De Guo} and Song, {Tan Jing} and Qun He and Ma, {Chun Gu} and Duan Ma and Song, {Hou Yan} and Tang, {Qi Qun}",

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doi = "10.1016/j.bbrc.2006.08.188",

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T1 - Lactacystin inhibits 3T3-L1 adipocyte differentiation through induction of CHOP-10 expression

AU - Li, Xi

AU - Huang, Hai Yan

AU - Chen, Jie Gen

AU - Jiang, Lin

AU - Liu, Hong Lei

AU - Liu, De Guo

AU - Song, Tan Jing

AU - He, Qun

AU - Ma, Chun Gu

AU - Ma, Duan

AU - Song, Hou Yan

AU - Tang, Qi Qun

PY - 2006/11/10

Y1 - 2006/11/10

N2 - Hormonal induction triggers a cascade leading to the expression of CCAAT/enhancer-binding protein(C/EBP)α and peroxisome proliferator-activated receptor (PPAR) γ, C/EBPα, and PPARγ turns on series of adipocyte genes that give rise to the adipocyte phenotype. Previous findings indicate that C/EBPβ, a transcriptional activator of the C/EBPα and PPARγ genes, is rapidly expressed after induction, but lacks DNA-binding activity and therefore cannot activate transcription of the C/EBPα and PPARγ genes early in the differentiation program. Acquisition of DNA-binding activity of C/EBPβ occurs when CHOP-10, a dominant-negative form of C/EBP family members, is down-regulated and becomes hyperphosphorylated as preadipocytes traverse the G1-S checkpoint of mitotic clonal expansion. Evidences are presented in this report that lactacystin, a proteasome inhibitor, up-regulated the CHOP-10 expression, blocked the DNA-binding activity of C/EBPβ, and subsequently inhibited MCE as well as adipocyte differentiation.

AB - Hormonal induction triggers a cascade leading to the expression of CCAAT/enhancer-binding protein(C/EBP)α and peroxisome proliferator-activated receptor (PPAR) γ, C/EBPα, and PPARγ turns on series of adipocyte genes that give rise to the adipocyte phenotype. Previous findings indicate that C/EBPβ, a transcriptional activator of the C/EBPα and PPARγ genes, is rapidly expressed after induction, but lacks DNA-binding activity and therefore cannot activate transcription of the C/EBPα and PPARγ genes early in the differentiation program. Acquisition of DNA-binding activity of C/EBPβ occurs when CHOP-10, a dominant-negative form of C/EBP family members, is down-regulated and becomes hyperphosphorylated as preadipocytes traverse the G1-S checkpoint of mitotic clonal expansion. Evidences are presented in this report that lactacystin, a proteasome inhibitor, up-regulated the CHOP-10 expression, blocked the DNA-binding activity of C/EBPβ, and subsequently inhibited MCE as well as adipocyte differentiation.

KW - C/EBPα

KW - C/EBPβ

KW - DNA-binding activity

KW - Mitotic clonal expansion

KW - PPARγ

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Lactacystin inhibits 3T3-L1 adipocyte differentiation through induction of CHOP-10 expression

Abstract

Keywords

ASJC Scopus subject areas

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