Lack of role of TGF-β1 in decreased lymphoproliferative response in HIV-1 infection

R. K. Chopra, N. B.K. Raj, J. P. Scally, W. R. Cappuccio, J. E. Nagel, A. J. Saah, J. B. Margolick

Research output: Contribution to journalArticle

Abstract

We investigated whether reduction of the phytohemagglutinin (PHA)-induced proliferative response of lymphocytes from HIV type-1 (HIV-1)-infected (HIV+) individuals could be explained by overproduction of transforming growth factor-β1 (TGF-β1), a strong inhibitor of T cell proliferation. PHA-stimulated PBMC from 40 HIV- and 42 HIV+ homosexual men from the Baltimore Center of the Multicenter AIDS Cohort Study (MACS) were studied using Northern blot analysis of expression of TGF-β1 mRNA and determining the effects of anti-TGF-β1 neutralizing antibody on PHA-induced proliferative responses. Compared to the HIV- donors, HIV+ donors did not show increased expression of TGF-β1 mRNA in unstimulated or PHA-stimulated PBMC. Furthermore, a neutralizing antibody to TGF-β1 did not reverse the decreased proliferative response of PBMC from HIV+ individuals to PHA or interleukin-2. These results indicate that TGF-β1 is not involved in T cell proliferation defects seen in HIV+ donors.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalClinical Immunology and Immunopathology
Volume69
Issue number1
DOIs
StatePublished - Oct 1993

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

Fingerprint Dive into the research topics of 'Lack of role of TGF-β1 in decreased lymphoproliferative response in HIV-1 infection'. Together they form a unique fingerprint.

  • Cite this