Lack of imprinting of three human cyclin-dependent kinase inhibitor genes

Gregory J. Cost, Jeffrey S. Thompson, Betty Anne Reichard, Jae Yong Lee, Andrew P Feinberg

Research output: Contribution to journalArticle

Abstract

Genomic imprinting is an epigenetic modification in the germline leading to parental allele-specific gene expression in somatic cells. We have previously found that imprinted genes can be abnormally expressed or silenced in tumors and that the cyclin-dependent kinase inhibitor (CKI) CDKN1C (p57(KIP2)) is normally imprinted, with preferential expression of the maternal allele. Here we analyze the imprinting status of three additional CKIs, the abnormal expression and/or chromosomal localization of which has been implicated in human malignancy: CDKN1A, CDKN1B, and CDAN2C. Allele- specific expression was examined by reverse transcription-PCR, using primers that span transcribed polymorphisms as well as exon/intron boundaries, to distinguish cDNA products from genomic DNA. Biallelic expression was observed for all three genes in both fetal and adult tissues. Thus, genomic imprinting is not a generalized feature of CKIs.

Original languageEnglish (US)
Pages (from-to)926-929
Number of pages4
JournalCancer Research
Volume57
Issue number5
StatePublished - 1997

Fingerprint

Cyclin-Dependent Kinases
Genomic Imprinting
Alleles
Cyclin-Dependent Kinase Inhibitor p57
Genes
Epigenomics
Introns
Reverse Transcription
Exons
Neoplasms
Fetus
Complementary DNA
Mothers
Gene Expression
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cost, G. J., Thompson, J. S., Reichard, B. A., Lee, J. Y., & Feinberg, A. P. (1997). Lack of imprinting of three human cyclin-dependent kinase inhibitor genes. Cancer Research, 57(5), 926-929.

Lack of imprinting of three human cyclin-dependent kinase inhibitor genes. / Cost, Gregory J.; Thompson, Jeffrey S.; Reichard, Betty Anne; Lee, Jae Yong; Feinberg, Andrew P.

In: Cancer Research, Vol. 57, No. 5, 1997, p. 926-929.

Research output: Contribution to journalArticle

Cost, GJ, Thompson, JS, Reichard, BA, Lee, JY & Feinberg, AP 1997, 'Lack of imprinting of three human cyclin-dependent kinase inhibitor genes', Cancer Research, vol. 57, no. 5, pp. 926-929.
Cost, Gregory J. ; Thompson, Jeffrey S. ; Reichard, Betty Anne ; Lee, Jae Yong ; Feinberg, Andrew P. / Lack of imprinting of three human cyclin-dependent kinase inhibitor genes. In: Cancer Research. 1997 ; Vol. 57, No. 5. pp. 926-929.
@article{c00d6709634444f2ba86bb4687a98da5,
title = "Lack of imprinting of three human cyclin-dependent kinase inhibitor genes",
abstract = "Genomic imprinting is an epigenetic modification in the germline leading to parental allele-specific gene expression in somatic cells. We have previously found that imprinted genes can be abnormally expressed or silenced in tumors and that the cyclin-dependent kinase inhibitor (CKI) CDKN1C (p57(KIP2)) is normally imprinted, with preferential expression of the maternal allele. Here we analyze the imprinting status of three additional CKIs, the abnormal expression and/or chromosomal localization of which has been implicated in human malignancy: CDKN1A, CDKN1B, and CDAN2C. Allele- specific expression was examined by reverse transcription-PCR, using primers that span transcribed polymorphisms as well as exon/intron boundaries, to distinguish cDNA products from genomic DNA. Biallelic expression was observed for all three genes in both fetal and adult tissues. Thus, genomic imprinting is not a generalized feature of CKIs.",
author = "Cost, {Gregory J.} and Thompson, {Jeffrey S.} and Reichard, {Betty Anne} and Lee, {Jae Yong} and Feinberg, {Andrew P}",
year = "1997",
language = "English (US)",
volume = "57",
pages = "926--929",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "5",

}

TY - JOUR

T1 - Lack of imprinting of three human cyclin-dependent kinase inhibitor genes

AU - Cost, Gregory J.

AU - Thompson, Jeffrey S.

AU - Reichard, Betty Anne

AU - Lee, Jae Yong

AU - Feinberg, Andrew P

PY - 1997

Y1 - 1997

N2 - Genomic imprinting is an epigenetic modification in the germline leading to parental allele-specific gene expression in somatic cells. We have previously found that imprinted genes can be abnormally expressed or silenced in tumors and that the cyclin-dependent kinase inhibitor (CKI) CDKN1C (p57(KIP2)) is normally imprinted, with preferential expression of the maternal allele. Here we analyze the imprinting status of three additional CKIs, the abnormal expression and/or chromosomal localization of which has been implicated in human malignancy: CDKN1A, CDKN1B, and CDAN2C. Allele- specific expression was examined by reverse transcription-PCR, using primers that span transcribed polymorphisms as well as exon/intron boundaries, to distinguish cDNA products from genomic DNA. Biallelic expression was observed for all three genes in both fetal and adult tissues. Thus, genomic imprinting is not a generalized feature of CKIs.

AB - Genomic imprinting is an epigenetic modification in the germline leading to parental allele-specific gene expression in somatic cells. We have previously found that imprinted genes can be abnormally expressed or silenced in tumors and that the cyclin-dependent kinase inhibitor (CKI) CDKN1C (p57(KIP2)) is normally imprinted, with preferential expression of the maternal allele. Here we analyze the imprinting status of three additional CKIs, the abnormal expression and/or chromosomal localization of which has been implicated in human malignancy: CDKN1A, CDKN1B, and CDAN2C. Allele- specific expression was examined by reverse transcription-PCR, using primers that span transcribed polymorphisms as well as exon/intron boundaries, to distinguish cDNA products from genomic DNA. Biallelic expression was observed for all three genes in both fetal and adult tissues. Thus, genomic imprinting is not a generalized feature of CKIs.

UR - http://www.scopus.com/inward/record.url?scp=0031019207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031019207&partnerID=8YFLogxK

M3 - Article

C2 - 9041196

AN - SCOPUS:0031019207

VL - 57

SP - 926

EP - 929

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 5

ER -