Lack of imprinting of three human cyclin-dependent kinase inhibitor genes

Gregory J. Cost, Jeffrey S. Thompson, Betty Anne Reichard, Jae Yong Lee, Andrew P. Feinberg

Research output: Contribution to journalArticlepeer-review

Abstract

Genomic imprinting is an epigenetic modification in the germline leading to parental allele-specific gene expression in somatic cells. We have previously found that imprinted genes can be abnormally expressed or silenced in tumors and that the cyclin-dependent kinase inhibitor (CKI) CDKN1C (p57(KIP2)) is normally imprinted, with preferential expression of the maternal allele. Here we analyze the imprinting status of three additional CKIs, the abnormal expression and/or chromosomal localization of which has been implicated in human malignancy: CDKN1A, CDKN1B, and CDAN2C. Allele- specific expression was examined by reverse transcription-PCR, using primers that span transcribed polymorphisms as well as exon/intron boundaries, to distinguish cDNA products from genomic DNA. Biallelic expression was observed for all three genes in both fetal and adult tissues. Thus, genomic imprinting is not a generalized feature of CKIs.

Original languageEnglish (US)
Pages (from-to)926-929
Number of pages4
JournalCancer Research
Volume57
Issue number5
StatePublished - 1997

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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