Lack of effect of ovariectomy on the metabolism of vitamin D and intestinal calcium-binding protein in female rats

H. Pavlovitch, T. L. Clemens, D. Laouari, J. L. O'Riordan, S. Balsan

Research output: Contribution to journalArticle

Abstract

The metabolism of 25-hydroxycholecalciferol (25-(OH)D3), plasma concentration of 1,25-dihydroxycholecalciferol (1,25-(OH)2D3) and the amount of calcium-binding protein (CaBP) in duodenal mucosa were determined in ovariectomized rats and were compared with data observed in normal age-matched cyclic rats. Sephadex LH-20 and high-pressure liquid chromatography were used for the study of the metabolism of 25-(OH)D3. The concentration of 1,25-(OH)2D3 in plasma and prolactin in serum were measured by radioimmunoassay. Calcium-binding protein in duodenal mucosa was determined immunologically using electroimmunodiffusion. The results showed that the lack of ovarian hormones and low prolactin levels observed in ovariectomized rats did not promote a significant change in the metabolism of 25-(OH)D3, in the levels of 1,25-(OH)2D3 in the circulation or in the amount of CaBP in duodenal mucosa. It is possible that the regulation of 25-(OH)D3 by sex hormones is restricted to the state of calcium stress such as during egg-laying in birds or pregnancy and lactation in mammals.

Original languageEnglish (US)
Pages (from-to)419-424
Number of pages6
JournalJournal of Endocrinology
Volume86
Issue number3
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Fingerprint Dive into the research topics of 'Lack of effect of ovariectomy on the metabolism of vitamin D and intestinal calcium-binding protein in female rats'. Together they form a unique fingerprint.

  • Cite this