Lack of effect of histamine H2-receptor antagonists on indocyanine green disposition measured by two methods

K. H. Donn, J. R. Powell, J. F. Rogers, J. R. Plachetka

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Abstract

Eleven healthy male volunteers were treated according to a randomized, crossover design with ranitidine (300 mg/day), cimetidine (1200 mg/day), or nothing for 48 hours. Ninety minutes after the 48-hour dose, each volunteer was given 0.5 mg/kg indocyanine green by iv bolus. Indocyanine green plasma concentrations were measured by the traditional spectrophotometric method at 800 nm and by HPLC simultaneously monitored at 214 and 656 nm. Neither histamine H2-receptor antagonist altered the disposition of indocyanine green. The mean (± S.D.) plasma clearance by the spectrophotometric method was 7.48 ± 2.07 (control), 7.15 ± 3.07 (ranitidine), and 6.88 ± 1.35 ml/min/kg (cimetidine). The power to detect a 20% change is 0.87. The spectrophotometric method generally produced a biexponential plasma concentration decay, whereas the HPLC method resulted in a monoexponential decay. Analysis of the 5- to 15-minute data by the conventional technique showed that although indocyanine green total plasma clearance was not significantly different for the 2 methods (p > 0.10), the volume of distribution was significantly greater (P <0.001) and the elimination rate constant was significantly smaller (P <0.001) for the spectrophotometric than the HPLC method. Although neither ranitidine nor cimetidine chronic administration alters indocyanine green disposition by either method, the absolute values of the pharmacokinetic parameters are dependent upon the analytical technique employed.

Original languageEnglish (US)
Pages (from-to)360-370
Number of pages11
JournalJournal of Clinical Pharmacology
Volume24
Issue number8-9
Publication statusPublished - 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Pharmacology (medical)

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