TY - JOUR
T1 - Lack of dependence on p53 for DNA double strand break repair of episomal vectors in human lymphoblasts
AU - Kohli, Manu
AU - Jorgensen, Timothy J.
N1 - Funding Information:
We gratefully acknowledge H. Tian and E. Wittmack for helpful discussions. This work was supported by Grant 9307-0016 from the National Aeronautics and Space Administration.
PY - 1999/11/2
Y1 - 1999/11/2
N2 - The p53 tumor suppressor gene has been shown to be involved in a variety of repair processes, and recent findings have suggested that p53 may be involved in DNA double strand break repair in irradiated cells. The role of p53 in DNA double strand break repair, however, has not been fully investigated. In this study, we have constructed a novel Epstein-Barr virus (EBV)-based shuttle vector, designated as pZEBNA, to explore the influence of p53 on DNA strand break repair in human lymphoblasts, since EBV-based vectors do not inactivate the p53 pathway. We have compared plasmid survival of irradiated, restriction enzyme linearized, and calf intestinal alkaline phosphatase (CIP)-treated pZEBNA with a Simian virus 40 (SV40)-based shuttle vector, pZ189, in TK6 (wild-type p53) and WTK1 (mutant p53) lymphoblasts and determined that p53 does not modulate DNA double strand break repair in these cell lines.
AB - The p53 tumor suppressor gene has been shown to be involved in a variety of repair processes, and recent findings have suggested that p53 may be involved in DNA double strand break repair in irradiated cells. The role of p53 in DNA double strand break repair, however, has not been fully investigated. In this study, we have constructed a novel Epstein-Barr virus (EBV)-based shuttle vector, designated as pZEBNA, to explore the influence of p53 on DNA strand break repair in human lymphoblasts, since EBV-based vectors do not inactivate the p53 pathway. We have compared plasmid survival of irradiated, restriction enzyme linearized, and calf intestinal alkaline phosphatase (CIP)-treated pZEBNA with a Simian virus 40 (SV40)-based shuttle vector, pZ189, in TK6 (wild-type p53) and WTK1 (mutant p53) lymphoblasts and determined that p53 does not modulate DNA double strand break repair in these cell lines.
KW - DNA repair
KW - Epstein-Barr virus
KW - P53
KW - Radiation
KW - SV40
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U2 - 10.1006/bbrc.1999.1575
DO - 10.1006/bbrc.1999.1575
M3 - Article
C2 - 10543995
AN - SCOPUS:0033517779
SN - 0006-291X
VL - 264
SP - 702
EP - 708
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -