Lack of dependence on p53 for DNA double strand break repair of episomal vectors in human lymphoblasts

Manu Kohli, Timothy J. Jorgensen

Research output: Contribution to journalArticle

Abstract

The p53 tumor suppressor gene has been shown to be involved in a variety of repair processes, and recent findings have suggested that p53 may be involved in DNA double strand break repair in irradiated cells. The role of p53 in DNA double strand break repair, however, has not been fully investigated. In this study, we have constructed a novel Epstein-Barr virus (EBV)-based shuttle vector, designated as pZEBNA, to explore the influence of p53 on DNA strand break repair in human lymphoblasts, since EBV-based vectors do not inactivate the p53 pathway. We have compared plasmid survival of irradiated, restriction enzyme linearized, and calf intestinal alkaline phosphatase (CIP)-treated pZEBNA with a Simian virus 40 (SV40)-based shuttle vector, pZ189, in TK6 (wild-type p53) and WTK1 (mutant p53) lymphoblasts and determined that p53 does not modulate DNA double strand break repair in these cell lines.

Original languageEnglish (US)
Pages (from-to)702-708
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume264
Issue number3
DOIs
StatePublished - Nov 2 1999

Keywords

  • DNA repair
  • Epstein-Barr virus
  • P53
  • Radiation
  • SV40

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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