Lack of correlation between bronchial late allergic reaction to Dermatophagoides pteronyssinus and in vitro immunoglobulin E reactivity to histamine-releasing factor derived from mononuclear cells

Ilona Kleine Budde, Christa E. Lopuhaa, Pleuni G. De Heer, Jacqueline M. Langdon, Susan M. MacDonald, Jaring S. Van der Zee, Rob C. Aalberse

Research output: Contribution to journalArticle

Abstract

Background: Activity of immunoglobulin (Ig)E-dependent histamine-releasing factor (HRF) is dependent on the IgE molecules bound to the surface of basophils. Sera capable of passively sensitizing basophils to release histamine to HRF were designated IgE+ sera. IgE+ and HRF have been suggested to play a role in late allergic reaction (LAR). Objective: The working hypothesis was tested that IgE+ induces a LAR. Further, activity of HRF produced by mononuclear cells (HRFmn) was compared with that of recombinant HRF p23. Methods: Atopic patients (n = 82) were bronchially provoked with Dermatophagoides pteronyssinus extract and the change in forced expiratory volume in 1 second was monitored. A LAR was defined as forced expiratory volume in 1 second as percentage of baseline <80% 4 to 10 hours after allergen challenge. The presence of HRF-responsive IgE in serum was determined using basophils sensitized in vitro by serum. Results: The presence of HRFmn-responsive IgE (IgEmn+) in serum was shown not be essential for a LAR: 63% of the patients with a LAR had no IgEmn+ in their serum. Further, 71% of patients with IgEmn+ did not have a LAR. HRFmn and recombinant HRF p23 were not equivalent in the bioassay: serum of 38 of 82 atopic patients sensitized basophils to release histamine to HRFmn, whereas this was found with serum of 1 of 82 patients to HRF p23. Conclusions: The results do not support the hypothesis that IgEmn+ induces a LAR, but do not exclude the alternative hypothesis that HRFs are released during a LAR and contribute to asthma severity.

Original languageEnglish (US)
Pages (from-to)606-612
Number of pages7
JournalAnnals of Allergy, Asthma and Immunology
Volume89
Issue number6
DOIs
StatePublished - Dec 1 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

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