Laboratory maintenance of rickettsia rickettsii

Nicole Ammerman, Magda Beier-Sexton, Abdu F. Azad

Research output: Contribution to journalReview article

Abstract

This unit includes protocols for the laboratory maintenance of the obligate intracellular bacterium Rickettsia rickettsii, including propagation in mammalian cell cultures, as isolation, counting, and storage procedures. Regulations for working with ettsii in biosafety level 3 containment are also discussed.

Original languageEnglish (US)
JournalCurrent Protocols in Microbiology
Issue numberSUPPL. 11
DOIs
Publication statusPublished - Dec 1 2008
Externally publishedYes

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Keywords

  • Cell culture techniques
  • Rickettsia
  • Rocky Mountain spotted fever
  • Vero cells

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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