Abstract
This unit includes protocols for the laboratory maintenance of the obligate intracellular bacterium Rickettsia rickettsii, including propagation in mammalian cell cultures, as isolation, counting, and storage procedures. Regulations for working with ettsii in biosafety level 3 containment are also discussed.
Original language | English (US) |
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Pages (from-to) | 3A.5.1-3A.5.21 |
Journal | Current Protocols in Microbiology |
Issue number | SUPPL. 11 |
DOIs | |
State | Published - 2008 |
Externally published | Yes |
Keywords
- Cell culture techniques
- Rickettsia
- Rocky Mountain spotted fever
- Vero cells
ASJC Scopus subject areas
- Parasitology
- Microbiology
- Virology