Laboratory analyses in the diagnosis of human allergic disease

Research output: Contribution to journalArticlepeer-review

Abstract

The clinical immunology laboratory provides support to the allergist in the diagnosis and management of human allergic diseases. Following a clinical history, the detection of allergen-specific immunoglobulin E (IgE) in serum can be useful in the definitive diagnosis of an IgE antibody-mediated hypersensitivity. Total serum IgE, the multiallergen screen, and mast cell tryptase are less commonly measured analytes in the work-up of the allergic patient. More recently, the clinical laboratory has employed monoclonal antibody based-enzyme immunoassays to quantify dust mite, cat, dog, and cockroach indicator aeroallergen levels in indoor home and work environments. Levels of allergenic proteins in environmental and medical/consumer products are measured by other assays such as the competitive inhibition radioallergosorbent test. Methods of quantification, internal quality control, and external proficiency testing programs are emphasised in this report.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalMethods: A Companion to Methods in Enzymology
Volume13
Issue number1
DOIs
StatePublished - Sep 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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