TY - JOUR
T1 - Labetalol analysis in human plasma using liquid chromatography with electrochemical detection
T2 - Application to pharmacokinetic studies
AU - Abernethy, Darrel R.
AU - Todd, Elizabeth L.
AU - Egan, Josephine L.
AU - Carrum, George
PY - 1986/8/1
Y1 - 1986/8/1
N2 - Labetalol determination in human plasma by a sensitive (to 2.5 ng/ml) and selective method using liquid chromatography with electrochemical detection is described. Plasma is extracted with diethyl ether under mildly basic (pH 9) conditions, back-extracted into an aqueous acidic buffer, then injected directly on column. Standard curves using propranolol as an internal standard are linear for concentrations from 2.5 to 200 ng/ml. Within-day and between-day reproducibility is satisfactory with coefficient of variation less than 8% for all concentrations. Sample recovery from the extraction is complete at all concentrations. Utility of the method is demonstrated by a pharmacokinetic study in a hypertensive volunteer who received 43.75 mg labetalol by 10 minute intravenous infusion.
AB - Labetalol determination in human plasma by a sensitive (to 2.5 ng/ml) and selective method using liquid chromatography with electrochemical detection is described. Plasma is extracted with diethyl ether under mildly basic (pH 9) conditions, back-extracted into an aqueous acidic buffer, then injected directly on column. Standard curves using propranolol as an internal standard are linear for concentrations from 2.5 to 200 ng/ml. Within-day and between-day reproducibility is satisfactory with coefficient of variation less than 8% for all concentrations. Sample recovery from the extraction is complete at all concentrations. Utility of the method is demonstrated by a pharmacokinetic study in a hypertensive volunteer who received 43.75 mg labetalol by 10 minute intravenous infusion.
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U2 - 10.1080/01483918608074140
DO - 10.1080/01483918608074140
M3 - Article
AN - SCOPUS:0022762717
SN - 0148-3919
VL - 9
SP - 2153
EP - 2163
JO - Journal of Liquid Chromatography
JF - Journal of Liquid Chromatography
IS - 10
ER -