Labetalol analysis in human plasma using liquid chromatography with electrochemical detection: Application to pharmacokinetic studies

Darrel R. Abernethy, Elizabeth L. Todd, Josephine L. Egan, George Carrum

Research output: Contribution to journalArticlepeer-review

Abstract

Labetalol determination in human plasma by a sensitive (to 2.5 ng/ml) and selective method using liquid chromatography with electrochemical detection is described. Plasma is extracted with diethyl ether under mildly basic (pH 9) conditions, back-extracted into an aqueous acidic buffer, then injected directly on column. Standard curves using propranolol as an internal standard are linear for concentrations from 2.5 to 200 ng/ml. Within-day and between-day reproducibility is satisfactory with coefficient of variation less than 8% for all concentrations. Sample recovery from the extraction is complete at all concentrations. Utility of the method is demonstrated by a pharmacokinetic study in a hypertensive volunteer who received 43.75 mg labetalol by 10 minute intravenous infusion.

Original languageEnglish (US)
Pages (from-to)2153-2163
Number of pages11
JournalJournal of Liquid Chromatography
Volume9
Issue number10
DOIs
StatePublished - Aug 1 1986

ASJC Scopus subject areas

  • Molecular Medicine

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