Labeling of a protein with fluorophores using maleimide derivitization

Jagpreet S. Nanda, Jon R. Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Maleimide derivitization of a protein is an essential tool for putting probes such as fluorescent labels at different sites within a polypeptide chain. This allows one to better understand protein-protein or protein-nucleic acid interactions using various biophysical approaches such as fluorescence anisotropy and fluorescence resonance energy transfer (FRET).

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part A
PublisherAcademic Press Inc.
Pages79-86
Number of pages8
ISBN (Print)9780124200708
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Enzymology
Volume536
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Cysteines
  • Efficiency of labeling calculation
  • Fluorophores
  • Maleimide derivitization
  • Maleimide modification
  • Protein labeling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Nanda, J. S., & Lorsch, J. R. (2014). Labeling of a protein with fluorophores using maleimide derivitization. In Laboratory Methods in Enzymology: Protein Part A (pp. 79-86). (Methods in Enzymology; Vol. 536). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420070-8.00007-6