Labeling of a protein with fluorophores using maleimide derivitization

Jagpreet S. Nanda, Jon R. Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Maleimide derivitization of a protein is an essential tool for putting probes such as fluorescent labels at different sites within a polypeptide chain. This allows one to better understand protein-protein or protein-nucleic acid interactions using various biophysical approaches such as fluorescence anisotropy and fluorescence resonance energy transfer (FRET).

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages79-86
Number of pages8
Volume536
ISBN (Print)9780124200708
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume536
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Fluorophores
Labeling
Proteins
Fluorescence Resonance Energy Transfer
Fluorescence Polarization
Nucleic Acids
Labels
Anisotropy
Fluorescence
Peptides
maleimide

Keywords

  • Cysteines
  • Efficiency of labeling calculation
  • Fluorophores
  • Maleimide derivitization
  • Maleimide modification
  • Protein labeling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Nanda, J. S., & Lorsch, J. R. (2014). Labeling of a protein with fluorophores using maleimide derivitization. In Methods in Enzymology (Vol. 536, pp. 79-86). (Methods in Enzymology; Vol. 536). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420070-8.00007-6

Labeling of a protein with fluorophores using maleimide derivitization. / Nanda, Jagpreet S.; Lorsch, Jon R.

Methods in Enzymology. Vol. 536 Academic Press Inc., 2014. p. 79-86 (Methods in Enzymology; Vol. 536).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nanda, JS & Lorsch, JR 2014, Labeling of a protein with fluorophores using maleimide derivitization. in Methods in Enzymology. vol. 536, Methods in Enzymology, vol. 536, Academic Press Inc., pp. 79-86. https://doi.org/10.1016/B978-0-12-420070-8.00007-6
Nanda JS, Lorsch JR. Labeling of a protein with fluorophores using maleimide derivitization. In Methods in Enzymology. Vol. 536. Academic Press Inc. 2014. p. 79-86. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-420070-8.00007-6
Nanda, Jagpreet S. ; Lorsch, Jon R. / Labeling of a protein with fluorophores using maleimide derivitization. Methods in Enzymology. Vol. 536 Academic Press Inc., 2014. pp. 79-86 (Methods in Enzymology).
@inbook{19d2242e424d49b6a8d34a29435ea427,
title = "Labeling of a protein with fluorophores using maleimide derivitization",
abstract = "Maleimide derivitization of a protein is an essential tool for putting probes such as fluorescent labels at different sites within a polypeptide chain. This allows one to better understand protein-protein or protein-nucleic acid interactions using various biophysical approaches such as fluorescence anisotropy and fluorescence resonance energy transfer (FRET).",
keywords = "Cysteines, Efficiency of labeling calculation, Fluorophores, Maleimide derivitization, Maleimide modification, Protein labeling",
author = "Nanda, {Jagpreet S.} and Lorsch, {Jon R.}",
year = "2014",
doi = "10.1016/B978-0-12-420070-8.00007-6",
language = "English (US)",
isbn = "9780124200708",
volume = "536",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "79--86",
booktitle = "Methods in Enzymology",

}

TY - CHAP

T1 - Labeling of a protein with fluorophores using maleimide derivitization

AU - Nanda, Jagpreet S.

AU - Lorsch, Jon R.

PY - 2014

Y1 - 2014

N2 - Maleimide derivitization of a protein is an essential tool for putting probes such as fluorescent labels at different sites within a polypeptide chain. This allows one to better understand protein-protein or protein-nucleic acid interactions using various biophysical approaches such as fluorescence anisotropy and fluorescence resonance energy transfer (FRET).

AB - Maleimide derivitization of a protein is an essential tool for putting probes such as fluorescent labels at different sites within a polypeptide chain. This allows one to better understand protein-protein or protein-nucleic acid interactions using various biophysical approaches such as fluorescence anisotropy and fluorescence resonance energy transfer (FRET).

KW - Cysteines

KW - Efficiency of labeling calculation

KW - Fluorophores

KW - Maleimide derivitization

KW - Maleimide modification

KW - Protein labeling

UR - http://www.scopus.com/inward/record.url?scp=84892413812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892413812&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-420070-8.00007-6

DO - 10.1016/B978-0-12-420070-8.00007-6

M3 - Chapter

C2 - 24423268

AN - SCOPUS:84892413812

SN - 9780124200708

VL - 536

T3 - Methods in Enzymology

SP - 79

EP - 86

BT - Methods in Enzymology

PB - Academic Press Inc.

ER -