Labeling cells with silver/dendrimer nanocomposites

Wojciech Lesniak, Xiangyang Shi, Anna Bielinska, Katarzyna Janczak, Kai Sun, James R. Baker, Lajos P. Balogh

Research output: Contribution to journalConference articlepeer-review

Abstract

We have developed water-soluble, biocompatible, fluorescent, and photostable silver/dendrimer nanocomposites that have a potential to be used for in vitro cell labeling. A PAMAM_E5.NH 2 dendrimer was used as a template to prepare first a silver-dendrimer complex in an aqueous solution at biologic pH=7.4. Conversion into nanocomposite was achieved by irradiating the solution of the [(Ag +) 25-PAMAM_E5.NH 2] complex by UV light to reduce the bound Ag + to zero-valent Ag 0 atoms, which were simultaneously trapped in the dendrimer network. Results indicate that the {(Ag 0) 25-PAMAM_E5.NH 2} silver/dendrimer nanocomposite forms positively charged single particles of 4-5 nm under the experimental conditions used. The dendrimer nanocomposite proved to be fluorescent. Toxicity testing of {(Ag 0) 25- PAMAM_E5.NH 2) nanocomposite revealed a behavior similar to the template dendrimer. Intracellular internalization of the silver nanocomposite and cell labeling capabilities was confirmed by confocal microscopy.

Original languageEnglish (US)
Article numberAA5.37
Pages (from-to)327-332
Number of pages6
JournalMaterials Research Society Symposium Proceedings
Volume845
StatePublished - Jun 20 2005
Externally publishedYes
EventNanoscale Materials Science in Biology and Medicine - Boston, MA, United States
Duration: Nov 28 2004Dec 2 2004

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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