Labeling cells with silver/dendrimer nanocomposites

Wojciech Lesniak, Xiangyang Shi, Anna Bielinska, Katarzyna Janczak, Kai Sun, James R. Baker, Lajos P. Balogh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed water-soluble, biocompatible, fluorescent, and photostable silver/dendrimer nanocomposites that have a potential to be used for in vitro cell labeling. A PAMAM_E5.NH 2 dendrimer was used as a template to prepare first a silver-dendrimer complex in an aqueous solution at biologic pH=7.4. Conversion into nanocomposite was achieved by irradiating the solution of the [(Ag +) 25-PAMAM_E5.NH 2] complex by UV light to reduce the bound Ag + to zero-valent Ag 0 atoms, which were simultaneously trapped in the dendrimer network. Results indicate that the {(Ag 0) 25-PAMAM_E5.NH 2} silver/dendrimer nanocomposite forms positively charged single particles of 4-5 nm under the experimental conditions used. The dendrimer nanocomposite proved to be fluorescent. Toxicity testing of {(Ag 0) 25- PAMAM_E5.NH 2) nanocomposite revealed a behavior similar to the template dendrimer. Intracellular internalization of the silver nanocomposite and cell labeling capabilities was confirmed by confocal microscopy.

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium Proceedings
EditorsC.T. Laurencin, E.A. Botchwey
Pages327-332
Number of pages6
Volume845
StatePublished - 2005
Externally publishedYes
EventNanoscale Materials Science in Biology and Medicine - Boston, MA, United States
Duration: Nov 28 2004Dec 2 2004

Other

OtherNanoscale Materials Science in Biology and Medicine
CountryUnited States
CityBoston, MA
Period11/28/0412/2/04

Fingerprint

Dendrimers
Silver
Labeling
Nanocomposites
Confocal microscopy
Ultraviolet radiation
Toxicity
Atoms
Water
Testing

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

Cite this

Lesniak, W., Shi, X., Bielinska, A., Janczak, K., Sun, K., Baker, J. R., & Balogh, L. P. (2005). Labeling cells with silver/dendrimer nanocomposites. In C. T. Laurencin, & E. A. Botchwey (Eds.), Materials Research Society Symposium Proceedings (Vol. 845, pp. 327-332). [AA5.37]

Labeling cells with silver/dendrimer nanocomposites. / Lesniak, Wojciech; Shi, Xiangyang; Bielinska, Anna; Janczak, Katarzyna; Sun, Kai; Baker, James R.; Balogh, Lajos P.

Materials Research Society Symposium Proceedings. ed. / C.T. Laurencin; E.A. Botchwey. Vol. 845 2005. p. 327-332 AA5.37.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lesniak, W, Shi, X, Bielinska, A, Janczak, K, Sun, K, Baker, JR & Balogh, LP 2005, Labeling cells with silver/dendrimer nanocomposites. in CT Laurencin & EA Botchwey (eds), Materials Research Society Symposium Proceedings. vol. 845, AA5.37, pp. 327-332, Nanoscale Materials Science in Biology and Medicine, Boston, MA, United States, 11/28/04.
Lesniak W, Shi X, Bielinska A, Janczak K, Sun K, Baker JR et al. Labeling cells with silver/dendrimer nanocomposites. In Laurencin CT, Botchwey EA, editors, Materials Research Society Symposium Proceedings. Vol. 845. 2005. p. 327-332. AA5.37
Lesniak, Wojciech ; Shi, Xiangyang ; Bielinska, Anna ; Janczak, Katarzyna ; Sun, Kai ; Baker, James R. ; Balogh, Lajos P. / Labeling cells with silver/dendrimer nanocomposites. Materials Research Society Symposium Proceedings. editor / C.T. Laurencin ; E.A. Botchwey. Vol. 845 2005. pp. 327-332
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