Label-free in vivo molecular imaging of underglycosylated mucin-1 expression in tumour cells

Xiaolei Song, Raag D. Airan, Dian Arifin, Amnon Bar-Shir, Deepak K. Kadayakkara, Guanshu Liu, Assaf A. Gilad, Peter C Van Zijl, Michael T Mcmahon, Jeff W Bulte

Research output: Contribution to journalArticle

Abstract

Alterations in mucin expression and glycosylation are associated with cancer development. Underglycosylated mucin-1 (uMUC1) is overexpressed in most malignant adenocarcinomas of epithelial origin (for example, colon, breast and ovarian cancer). Its counterpart MUC1 is a large polymer rich in glycans containing multiple exchangeable OH protons, which is readily detectable by chemical exchange saturation transfer (CEST) MRI. We show here that deglycosylation of MUC1 results in >75% reduction in CEST signal. Three uMUC1+ human malignant cancer cell lines overexpressing uMUC1 (BT20, HT29 and LS174T) show a significantly lower CEST signal compared with the benign human epithelial cell line MCF10A and the uMUC1- tumour cell line U87. Furthermore, we demonstrate that in vivo CEST MRI is able to make a distinction between LS174T and U87 tumour cells implanted in the mouse brain. These results suggest that the mucCEST MRI signal can be used as a label-free surrogate marker to non-invasively assess mucin glycosylation and tumour malignancy.

Original languageEnglish (US)
Article number6719
JournalNature Communications
Volume6
DOIs
StatePublished - Mar 30 2015

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Molecular imaging
Mucin-1
Molecular Imaging
Labels
Tumors
tumors
Cells
cultured cells
Magnetic resonance imaging
Glycosylation
saturation
cancer
Mucins
Neoplasms
Cell Line
breast
markers
brain
Polysaccharides
mice

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Label-free in vivo molecular imaging of underglycosylated mucin-1 expression in tumour cells. / Song, Xiaolei; Airan, Raag D.; Arifin, Dian; Bar-Shir, Amnon; Kadayakkara, Deepak K.; Liu, Guanshu; Gilad, Assaf A.; Van Zijl, Peter C; Mcmahon, Michael T; Bulte, Jeff W.

In: Nature Communications, Vol. 6, 6719, 30.03.2015.

Research output: Contribution to journalArticle

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AU - Arifin, Dian

AU - Bar-Shir, Amnon

AU - Kadayakkara, Deepak K.

AU - Liu, Guanshu

AU - Gilad, Assaf A.

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