L1 retrotransposition in nondividing and primary human somatic cells

Shuji Kubo, Maria Del Carmen Seleme, Harris S. Soifer, José Luis Garcia Perez, John V. Moran, Haig Kazazian, Noriyuki Kasahara

Research output: Contribution to journalArticle

Abstract

Whether long interspersed element-1 (L1 or LINE-1) retrotransposition can occur in quiescent, nondividing, and/or terminally differentiated somatic cells has remained an unanswered fundamental question in human genetics. Here, we used a ubiquitously active phosphoglycerate kinase-1 promoter to drive the expression of a highly active human L1 element from an adenovirus-L1 hybrid vector. This vector system achieved retrotransposition in up to 91% of actively growing immortalized cells, and we demonstrated that L1 retrotransposition can be suppressed by the reverse transcriptase inhibitor 3′-azido-3′- deoxythymidine. This adenovirus vector enabled efficient delivery of the L1 element into differentiated primary human somatic cells and G 1/S-arrested cells, resulting in retrotransposition in both cases; however, it was not detected in G0-arrested cells. Thus, these data indicate that L1 retrotransposition can occur in nondividing somatic cells.

Original languageEnglish (US)
Pages (from-to)8036-8041
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number21
DOIs
StatePublished - May 23 2006

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Long Interspersed Nucleotide Elements
Adenoviridae
Phosphoglycerate Kinase
Gastrin-Secreting Cells
Reverse Transcriptase Inhibitors
Zidovudine
Medical Genetics

Keywords

  • Adenovirus
  • Hybrid vector
  • LINE-1
  • Quiescent cell

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

L1 retrotransposition in nondividing and primary human somatic cells. / Kubo, Shuji; Del Carmen Seleme, Maria; Soifer, Harris S.; Perez, José Luis Garcia; Moran, John V.; Kazazian, Haig; Kasahara, Noriyuki.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 21, 23.05.2006, p. 8036-8041.

Research output: Contribution to journalArticle

Kubo, Shuji ; Del Carmen Seleme, Maria ; Soifer, Harris S. ; Perez, José Luis Garcia ; Moran, John V. ; Kazazian, Haig ; Kasahara, Noriyuki. / L1 retrotransposition in nondividing and primary human somatic cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 21. pp. 8036-8041.
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AU - Kazazian, Haig

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