Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis

Pan Duojia, Gerald M. Rubin

Research output: Contribution to journalArticle

Abstract

Notch and the disintegrin metalloprotease encoded by the kuzbanian (kuz) gene are both required for a lateral inhibition process during Drosophila neurogenesis. We show that a mutant KUZ protein lacking protease activity acts as a dominant-negative form in Drosophila. Expression of such a dominant-negative KUZ protein can perturb lateral inhibition in Xenopus, leading to the overproduction of primary neurons. This suggests an evolutionarily conserved role for KUZ. The Notch family of receptors are known to be processed into smaller forms under normal physiological conditions. We provide genetic and biochemical evidence that Notch is an in vivo substrate for the KUZ protease, and that this cleavage may be part of the normal biosynthesis of functional Notch proteins.

Original languageEnglish (US)
Pages (from-to)271-280
Number of pages10
JournalCell
Volume90
Issue number2
DOIs
StatePublished - Jul 25 1997
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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