The entire protein complement of a cell is termed the proteome. “Proteomics” is defined as the systematic expression of diverse properties of proteins in a cell. Proteomic methodologies can detect protein modifications, which occur after protein synthesis. The analysis of the proteom thus provides useful information, which can be used for the identification and screening of diagnostic markers, and is relevant for the understanding of tumor-progression. In past years, the most widely used tool of proteom-analysis has been 2D-gel electrophoresis. Today, new methods are available, which are based on biochip technology. High affinity surface-binding arrays can analyze epitopes of complex protein matrices and specify functional aspects of tumor-progression. After initial isolation, the separated proteins are identified by mass spectrometry based techniqiies such as MALDI (matrix assisted laser desorption ionization) or SELDI (surface enhanced laser desorption ionization)-TOF (time of flight). This review focuses on new developments in pro-teomics, including SELDI, and describes applications of these methods for the search of new “protein signatures” in cancer research. It is expected that the advancements of proteomdcs-techniques will help to classify human cancer by molecular rather than morphological characteristics.
ASJC Scopus subject areas
- Clinical Biochemistry
- Medical Laboratory Technology
- Biochemistry, medical