Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice1

Dinani Matoso Filho Armstrong, Gautam Sikka, Anderson da Costa Armstrong, Karen Ruggeri Saad, William Rodrigues de Freitas, Dan E Berkowitz, Djalma José Fagundes, Lakshmi Santhanam, Murched Omar Taha

Research output: Contribution to journalArticle

Abstract

PURPOSE: To determine whether the absence of transglutaminase 2 enzyme (TG2) in TG2 knockout mice (TG2-/-) protect them against early age-related functional and histological arterial changes. METHODS: Pulse wave velocity (PWV) was measured using non-invasive Doppler and mean arterial pressure (MAP) was measured in awake mice using tail-cuff system. Thoracic aortas were excised for evaluation of endothelial dependent vasodilation (EDV) by wire myography, as well as histological analyses. RESULTS: PWV and MAP were similar in TG2-/-mice to age-matched wild type (WT) control mice. Old WT mice exhibited a markedly attenuated EDV as compared to young WT animals. The TG2-/-young and old mice had enhanced EDV responses (p<0.01) as compared to WT mice. There was a significant increase in TG2 crosslinks by IHC in WT old group compared to Young, with no stain in the TG2-/-animals. Optical microscopy examination of Old WT mice aorta showed thinning and fragmentation of elastic laminae. Young WT mice, old and young TG2-/-mice presented regularly arranged and parallel elastic laminae of the tunica media. CONCLUSION: The genetic suppression of TG2 delays the age-induced endothelial dysfunction and histological modifications.

Original languageEnglish (US)
Pages (from-to)991-999
Number of pages9
JournalActa Cirurgica Brasileira
Volume33
Issue number11
DOIs
StatePublished - Nov 1 2018

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Blood Vessels
Enzymes
Vasodilation
Pulse Wave Analysis
Arterial Pressure
Myography
Genetic Suppression
Tunica Media
transglutaminase 2
Wild Animals
Thoracic Aorta
Knockout Mice
Aorta
Tail
Microscopy
Coloring Agents

ASJC Scopus subject areas

  • Surgery

Cite this

Armstrong, D. M. F., Sikka, G., Armstrong, A. D. C., Saad, K. R., Freitas, W. R. D., Berkowitz, D. E., ... Taha, M. O. (2018). Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice1. Acta Cirurgica Brasileira, 33(11), 991-999. https://doi.org/10.1590/s0102-865020180110000006

Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice1. / Armstrong, Dinani Matoso Filho; Sikka, Gautam; Armstrong, Anderson da Costa; Saad, Karen Ruggeri; Freitas, William Rodrigues de; Berkowitz, Dan E; Fagundes, Djalma José; Santhanam, Lakshmi; Taha, Murched Omar.

In: Acta Cirurgica Brasileira, Vol. 33, No. 11, 01.11.2018, p. 991-999.

Research output: Contribution to journalArticle

Armstrong, DMF, Sikka, G, Armstrong, ADC, Saad, KR, Freitas, WRD, Berkowitz, DE, Fagundes, DJ, Santhanam, L & Taha, MO 2018, 'Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice1', Acta Cirurgica Brasileira, vol. 33, no. 11, pp. 991-999. https://doi.org/10.1590/s0102-865020180110000006
Armstrong DMF, Sikka G, Armstrong ADC, Saad KR, Freitas WRD, Berkowitz DE et al. Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice1. Acta Cirurgica Brasileira. 2018 Nov 1;33(11):991-999. https://doi.org/10.1590/s0102-865020180110000006
Armstrong, Dinani Matoso Filho ; Sikka, Gautam ; Armstrong, Anderson da Costa ; Saad, Karen Ruggeri ; Freitas, William Rodrigues de ; Berkowitz, Dan E ; Fagundes, Djalma José ; Santhanam, Lakshmi ; Taha, Murched Omar. / Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice1. In: Acta Cirurgica Brasileira. 2018 ; Vol. 33, No. 11. pp. 991-999.
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