Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice1

Dinani Matoso Filho Armstrong, Gautam Sikka, Anderson da Costa Armstrong, Karen Ruggeri Saad, William Rodrigues de Freitas, Dan E Berkowitz, Djalma José Fagundes, Lakshmi Santhanam, Murched Omar Taha

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PURPOSE: To determine whether the absence of transglutaminase 2 enzyme (TG2) in TG2 knockout mice (TG2-/-) protect them against early age-related functional and histological arterial changes. METHODS: Pulse wave velocity (PWV) was measured using non-invasive Doppler and mean arterial pressure (MAP) was measured in awake mice using tail-cuff system. Thoracic aortas were excised for evaluation of endothelial dependent vasodilation (EDV) by wire myography, as well as histological analyses. RESULTS: PWV and MAP were similar in TG2-/-mice to age-matched wild type (WT) control mice. Old WT mice exhibited a markedly attenuated EDV as compared to young WT animals. The TG2-/-young and old mice had enhanced EDV responses (p<0.01) as compared to WT mice. There was a significant increase in TG2 crosslinks by IHC in WT old group compared to Young, with no stain in the TG2-/-animals. Optical microscopy examination of Old WT mice aorta showed thinning and fragmentation of elastic laminae. Young WT mice, old and young TG2-/-mice presented regularly arranged and parallel elastic laminae of the tunica media. CONCLUSION: The genetic suppression of TG2 delays the age-induced endothelial dysfunction and histological modifications.

Original languageEnglish (US)
Pages (from-to)991-999
Number of pages9
JournalActa Cirurgica Brasileira
Issue number11
StatePublished - Nov 1 2018

ASJC Scopus subject areas

  • Surgery

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    Armstrong, D. M. F., Sikka, G., Armstrong, A. D. C., Saad, K. R., Freitas, W. R. D., Berkowitz, D. E., Fagundes, D. J., Santhanam, L., & Taha, M. O. (2018). Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice1. Acta Cirurgica Brasileira, 33(11), 991-999.