Knockdown of transglutaminase-2 prevents early age-induced vascular changes in mice

Dinani Matoso Filho Armstrong, Gautam Sikka, Anderson da Costa Armstrong, Karen Ruggeri Saad, William Rodrigues de Freitas, Dan E Berkowitz, Djalma José Fagundes, Lakshmi Santhanam, Murched Omar Taha

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Purpose: To determine whether the absence of transglutaminase 2 enzyme (TG2) in TG2 knockout mice (TG2 -/- ) protect them against early age-related functional and histological arterial changes. Methods: Pulse wave velocity (PWV) was measured using non-invasive Doppler and mean arterial pressure (MAP) was measured in awake mice using tail-cuff system. Thoracic aortas were excised for evaluation of endothelial dependent vasodilation (EDV) by wire myography, as well as histological analyses. Results: PWV and MAP were similar in TG2 -/- mice to age-matched wild type (WT) control mice. Old WT mice exhibited a markedly attenuated EDV as compared to young WT animals. The TG2 -/- young and old mice had enhanced EDV responses (p<0.01) as compared to WT mice. There was a significant increase in TG2 crosslinks by IHC in WT old group compared to Young, with no stain in the TG2 -/- animals. Optical microscopy examination of Old WT mice aorta showed thinning and fragmentation of elastic laminae. Young WT mice, old and young TG2 -/- mice presented regularly arranged and parallel elastic laminae of the tunica media. Conclusion: The genetic suppression of TG2 delays the age-induced endothelial dysfunction and histological modifications.

Original languageEnglish (US)
Pages (from-to)991-999
Number of pages9
JournalActa cirurgica brasileira
Volume33
Issue number11
DOIs
StatePublished - Nov 1 2018

Keywords

  • Aging
  • Endothelium-dependent relaxing factors
  • Mice
  • Transglutaminases

ASJC Scopus subject areas

  • Surgery

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