Knock in of the AKT1 E17K mutation in human breast epithelial cells does not recapitulate oncogenic PIK3CA mutations

J. Lauring, D. P. Cosgrove, S. Fontana, J. P. Gustin, H. Konishi, A. M. Abukhdeir, J. P. Garay, M. Mohseni, G. M. Wang, M. J. Higgins, D. Gorkin, M. Reis, B. Vogelstein, K. Polyak, M. Cowherd, P. J. Buckhaults, B. H. Park

Research output: Contribution to journalArticlepeer-review

Abstract

An oncogenic mutation (G49A:E17K) in the AKT1 gene has been described recently in human breast, colon, and ovarian cancers. The low frequency of this mutation and perhaps other selective pressures have prevented the isolation of human cancer cell lines that harbor this mutation thereby limiting functional analysis. Here, we create a physiologic in vitro model to study the effects of this mutation by using somatic cell gene targeting using the nontumorigenic human breast epithelial cell line, MCF10A. Surprisingly, knock in of E17K into the AKT1 gene had minimal phenotypic consequences and importantly, did not recapitulate the biochemical and growth characteristics seen with somatic cell knock in of PIK3CA hotspot mutations. These results suggest that mutations in critical genes within the PI3-kinase (PI3K) pathway are not functionally equivalent, and that other cooperative genetic events may be necessary to achieve oncogenic PI3K pathway activation in cancers that contain the AKT1 E17K mutation.

Original languageEnglish (US)
Pages (from-to)2337-2345
Number of pages9
JournalOncogene
Volume29
Issue number16
DOIs
StatePublished - Apr 2010

Keywords

  • Akt1
  • Breast epithelial cells
  • Gene targeting
  • Knock in
  • Oncogene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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