Kinetoplast DNA maxicircles: Networks within networks

Research output: Contribution to journalArticle

Abstract

Kinetoplast DNA (kDNA), the mitochondrial DNA of trypanosomes, is an enormous network of interlocked minicircles and maxicircles. We selectively removed minicircles from Trypanosoma equiperdum kDNA networks by restriction enzyme cleavage. Maxicircles remained in aggregates that were resistant to protease or RNase and contained no residual minicircles, but were resolved into circular monomers by topoisomerase II. Maxicircles thus form independent catenanes within kDNA networks. Heterogeneity in the size, composition, and organization of maxicircle catenanes reflects changes that occur during kDNA replication. The rosette-like arrangement of maxicircle catenanes is distinctly different from that of minicircle catenanes. Trypanosome kDNA networks reveal unique topological complexity: they are composed of entirely dissimilar catenanes that are in turn extensively interlocked with one another.

Original languageEnglish (US)
Pages (from-to)7809-7813
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number16
DOIs
StatePublished - Aug 15 1993

Keywords

  • Topology
  • Trypanosomes

ASJC Scopus subject areas

  • General

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