TY - JOUR
T1 - Kinetics of low-dose intravenous antipyrine
T2 - Use of liquid chromatography
AU - Greenblatt, D. J.
AU - Abernethy, D. R.
AU - Divoll, M.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1983
Y1 - 1983
N2 - Rapid and sensitive quantitation of antipyrine in plasma is achieved by high-presure liquid chromatography. Antipyrine and phenacetin, the internal standard, are readily extracted from alkalinized plasma into ethyl acetate. After evaporation of the organic solvent, the redissolved residue is chromatographed using a reverse-phase C-18 column. The sensitivity limit is approximately 0.25 μg antipyrine per ml plasma, with a coefficient of variation for identical samples not exceeding 3%. Using the automated sampling system, one person can analyze up to 100 samples per day. The method is reliable and sensitive enough to allow human pharmacokinetic studies of antipyrine using doses considerably less than utilized in previous studies. The disposition kinetics of a single i.v. dose of antipyrine in four human volunteers were essentially identical over a 5-fold ranges of doses.
AB - Rapid and sensitive quantitation of antipyrine in plasma is achieved by high-presure liquid chromatography. Antipyrine and phenacetin, the internal standard, are readily extracted from alkalinized plasma into ethyl acetate. After evaporation of the organic solvent, the redissolved residue is chromatographed using a reverse-phase C-18 column. The sensitivity limit is approximately 0.25 μg antipyrine per ml plasma, with a coefficient of variation for identical samples not exceeding 3%. Using the automated sampling system, one person can analyze up to 100 samples per day. The method is reliable and sensitive enough to allow human pharmacokinetic studies of antipyrine using doses considerably less than utilized in previous studies. The disposition kinetics of a single i.v. dose of antipyrine in four human volunteers were essentially identical over a 5-fold ranges of doses.
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M3 - Article
C2 - 6840925
AN - SCOPUS:0020659648
SN - 0174-4879
VL - 21
SP - 51
EP - 55
JO - International Journal of Clinical Pharmacology Therapy and Toxicology
JF - International Journal of Clinical Pharmacology Therapy and Toxicology
IS - 2
ER -