Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection

C. Graziosi, K. R. Gantt, M. Vaccarezza, J. F. Demarest, M. Daucher, M. S. Saag, G. M. Shaw, Thomas C Quinn, O. J. Cohen, C. C. Welbon, G. Pantaleo, A. S. Fauci

Research output: Contribution to journalArticle

Abstract

In the present study, we have determined the kinetics of constitutive expression of a panel of cytokines [interleukin (IL) 2, IL-4, IL-6, IL-10, interferon γ (IFN-γ), and tumor necrosis factor α (TNF-α)] in sequential peripheral blood mononuclear cell samples from nine individuals with primary human immunodeficiency virus infection. Expression of IL-2 and IL-4 was barely detected in peripheral blood mononuclear cells. However, substantial levels of IL-2 expression were found in mononuclear cells isolated from lymph node. Expression of IL-6 was detected in only three of nine patients, and IL- 6 expression was observed when transition from the acute to the chronic phase had already occurred. Expression of IL-10 and TNF-α was consistently observed in all patients tested, and levels of both cytokines were either stable or progressively increased over time. Similar to IL-10 and TNF-α, IFN-γ expression was detected in all patients; however, in five of nine patients, IFN-γ expression peaked very early during primary infection. The early peak in IFN-γ expression coincided with oligoclonal expansions of CD8+ T cells in five of six patients, and CD8+ T cells mostly accounted for the expression of this cytokine. These results indicate that high levels of expression of proinflammatory cytokines are associated with primary infection and that the cytokine response during this phase of infection is strongly influenced by oligoclonal expansions of CD8+ T cells.

Original languageEnglish (US)
Pages (from-to)4386-4391
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number9
DOIs
StatePublished - Apr 30 1996
Externally publishedYes

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Virus Diseases
HIV-1
Cytokines
Interleukin-10
Interleukin-2
Interleukin-6
T-Lymphocytes
Interleukin-4
Blood Cells
Infection
Interferons
Tumor Necrosis Factor-alpha
Lymph Nodes
HIV

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection. / Graziosi, C.; Gantt, K. R.; Vaccarezza, M.; Demarest, J. F.; Daucher, M.; Saag, M. S.; Shaw, G. M.; Quinn, Thomas C; Cohen, O. J.; Welbon, C. C.; Pantaleo, G.; Fauci, A. S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 9, 30.04.1996, p. 4386-4391.

Research output: Contribution to journalArticle

Graziosi, C, Gantt, KR, Vaccarezza, M, Demarest, JF, Daucher, M, Saag, MS, Shaw, GM, Quinn, TC, Cohen, OJ, Welbon, CC, Pantaleo, G & Fauci, AS 1996, 'Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection', Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 9, pp. 4386-4391. https://doi.org/10.1073/pnas.93.9.4386
Graziosi, C. ; Gantt, K. R. ; Vaccarezza, M. ; Demarest, J. F. ; Daucher, M. ; Saag, M. S. ; Shaw, G. M. ; Quinn, Thomas C ; Cohen, O. J. ; Welbon, C. C. ; Pantaleo, G. ; Fauci, A. S. / Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 9. pp. 4386-4391.
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