Kinetics characterization of ligand–receptor interactions using oblique–incidence reflectivity difference method

Shuang Liu, Guozhen Yang, Huibin Lu, Heng Zhu

Research output: Contribution to journalArticlepeer-review

Abstract

The oblique-incidence reflectivity difference (OIRD) method is a novel optical biosensor which has recently been applied to label-free, real-time detection of molecular interactions in microarray format using a functionalized glass substrate. The capability of the OIRD to independently monitor association and dissociation kinetics enables real-time profiling of ligand–receptor interactions. The microarray format of the OIRD method makes it a practical platform for high-throughput, system-wide affinity profiling of the interactions of proteins, DNA, or whole cells with a diverse set of biomolecules.

Original languageEnglish (US)
Pages (from-to)111-118
Number of pages8
JournalMethods in Pharmacology and Toxicology
Volume53
DOIs
StatePublished - 2015

Keywords

  • Binding kinetics
  • Biomolecular interactions
  • High-throughput
  • Label-free
  • incidence reflectivity difference (OIRD)

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Kinetics characterization of ligand–receptor interactions using oblique–incidence reflectivity difference method'. Together they form a unique fingerprint.

Cite this