The oblique-incidence reflectivity difference (OIRD) method is a novel optical biosensor which has recently been applied to label-free, real-time detection of molecular interactions in microarray format using a functionalized glass substrate. The capability of the OIRD to independently monitor association and dissociation kinetics enables real-time profiling of ligand–receptor interactions. The microarray format of the OIRD method makes it a practical platform for high-throughput, system-wide affinity profiling of the interactions of proteins, DNA, or whole cells with a diverse set of biomolecules.
- Binding kinetics
- Biomolecular interactions
- incidence reflectivity difference (OIRD)
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmacology (medical)