Kinetics characterization of ligand-receptor interactions using oblique-incidence reflectivity difference method

Shuang Liu, Guozhen Yang, Huibin Lu, Heng Zhu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The oblique-incidence reflectivity difference (OIRD) method is a novel optical biosensor which has recently been applied to label-free, real-time detection of molecular interactions in microarray format using a functionalized glass substrate. The capability of the OIRD to independently monitor association and dissociation kinetics enables real-time profiling of ligand-receptor interactions. The microarray format of the OIRD method makes it a practical platform for high-throughput, system-wide affinity profiling of the interactions of proteins, DNA, or whole cells with a diverse set of biomolecules.

Original languageEnglish (US)
Title of host publicationLabel-Free Biosensor Methods in Drug Discovery
PublisherSpringer New York
Pages111-118
Number of pages8
ISBN (Electronic)9781493926176
ISBN (Print)9781493926169
DOIs
StatePublished - Apr 6 2015

Keywords

  • Binding kinetics
  • Biomolecular interactions
  • High-throughput
  • Label-free
  • Oblique- incidence reflectivity difference (OIRD)

ASJC Scopus subject areas

  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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