Kinetic expression of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) during embryonic stem cell differentiation

Zong Jin Li, Zack Z. Wang, Yi Zhou Zheng, Bin Xu, Ren Chi Yang, David T. Scadden, Zhong Chao Han

Research output: Contribution to journalArticle

Abstract

Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is widely used as a marker during vasculogenesis and angiogenesis from embryonic stem (ES) cells. However, the expression of PECAM-1 isoforms in ES cells has not been determined. The present study was designed to determine the role of PECAM-1 isoforms during in vitro endothelial differentiation of ES cells. It was found that undifferentiated ES cells expressed high level of PECAM-1, which primarily located at cell-cell junction, but the expression of PECAM-1 was sharply down-regulated during early ES cell differentiation. In addition, undifferentiated ES cells were found the expressed all eight known alternatively spliced PECAM-1 isoforms, among them the expression of PECAM-1 isoforms lacking exon 15 or 14&15 was predominant. Quantitative analysis revealed a significant increase in the expression of PECAM-1 isoform lacking exon 12&14&15 as vascular development of ES cells. These results indicate a constitutive expression of PECAM-1 in undifferentiated murine ES cells and suggest a developmental role of PECAM-1 isoform changes during vasculogenesis and angiogenesis.

Original languageEnglish (US)
Pages (from-to)559-570
Number of pages12
JournalJournal of cellular biochemistry
Volume95
Issue number3
DOIs
StatePublished - Jun 1 2005

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Keywords

  • Alternative splicing
  • Angiogenesis
  • Embryonic stem cells
  • Endothelial differentiation
  • PECAM-1/CD31
  • Vasculogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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