Kinesin-mediated vesicular transport in a biochemically defined assay.

R. Urrutia, D. B. Murphy, B. Kachar, M. A. McNiven

Research output: Contribution to journalArticlepeer-review

Abstract

Here we have described simple and reproducible methods to observe kinesin-mediated vesicle and microtubule movements under defined conditions using video microscopy. We are optimistic that this assay will provide a useful tool to study kinesin function, regulation, and dynamic physical interactions with membranous organelles and microtubules.

Original languageEnglish (US)
Pages (from-to)253-266
Number of pages14
JournalMethods in Cell Biology
Volume39
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)
  • Cell Biology

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