Kinase-independent functions of TEL1 in telomere maintenance

Yunmei Ma, Carol W. Greider

Research output: Contribution to journalArticle

Abstract

TEL1 is important in Saccharomyces cerevisiae telomere maintenance, and its kinase activity is required. Tel1p associates with telomeres in vivo, is enriched at short telomeres, and enhances the binding of telomerase components to short telomeres. However, it is unclear how the kinase activity and telomere association contribute to Tel1p's overall function in telomere length maintenance. To investigate this question, we generated a set of single point mutants and a double point mutant (tel1KD) of Tel1p that were kinase deficient and two Xrs2p mutants that failed to bind Tel1p. Using these separation-of-function alleles in a de novo telomere elongation assay, we found, surprisingly, that the tel1KD allele and xrs2 C-terminal mutants were both partially functional. Combining the tel1KD and xrs2 C-terminal mutants had an additive effect and resembled the TEL1 null (tel1Δ) phenotype. These data indicate that Tel1p has two separate functions in telomere maintenance and that the Xrs2p-dependent recruitment of Tel1p to telomeres plays an important role even in the absence of its kinase activity.

Original languageEnglish (US)
Pages (from-to)5193-5202
Number of pages10
JournalMolecular and cellular biology
Volume29
Issue number18
DOIs
StatePublished - Sep 1 2009

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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