Killer artificial antigen presenting cells (kaAPC) for efficient in vitro depletion of human antigen-specific T cells

Christian Schütz, Martin Fleck, Jonathan P. Schneck, Mathias Oelke

Research output: Contribution to journalArticlepeer-review

Abstract

Current treatment of T cell mediated autoimmune diseases relies mostly on strategies of global immunosuppression, which, in the long term, is accompanied by adverse side effects such as a reduced ability to control infections or malignancies. Therefore, new approaches need to be developed that target only the disease mediating cells and leave the remaining immune system intact. Over the past decade a variety of cell based immunotherapy strategies to modulate T cell mediated immune responses have been developed. Most of these approaches rely on tolerance-inducing antigen presenting cells (APC). However, in addition to being technically difficult and cumbersome, such cell-based approaches are highly sensitive to cytotoxic T cell responses, which limits their therapeutic capacity. Here we present a protocol for the generation of non-cellular killer artificial antigen presenting cells (KaAPC), which allows for the depletion of pathologic T cells while leaving the remaining immune system untouched and functional. KaAPC is an alternative solution to cellular immunotherapy which has potential for treating autoimmune diseases and allograft rejections by regulating undesirable T cell responses in an antigen specific fashion.

Original languageEnglish (US)
Article numbere51859
JournalJournal of Visualized Experiments
Issue number90
DOIs
StatePublished - Aug 11 2014

Keywords

  • Apoptosis
  • Autoimmunity
  • Fas/FasL
  • HLA-A2-ig
  • Immunology
  • Issue 90
  • KaAPC

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint

Dive into the research topics of 'Killer artificial antigen presenting cells (kaAPC) for efficient in vitro depletion of human antigen-specific T cells'. Together they form a unique fingerprint.

Cite this