Killer artificial antigen-presenting cells: A novel strategy to delete specific T cells

Christian Schütz, Martin Fleck, Andreas Mackensen, Alessia Zoso, Dagmar Halbritter, Jonathan P. Schneck, Mathias Oelke

Research output: Contribution to journalArticle

Abstract

Several cell-based immunotherapy strategies have been developed to specifically modulate T cell-mediated immune responses. These methods frequently rely on the utilization of tolerogenic cell-based antigen-presenting cells (APCs). However, APCs are highly sensitive to cytotoxic T-cell responses, thus limiting their therapeutic capacity. Here, we describe a novel bead-based approach to modulate T-cell responses in an antigen-specific fashion. We have generated killer artificial APCs (κaAPCs) by coupling an apoptosis-inducing α-Fas (CD95) IgM mAb together with HLA-A 2 Ig molecules onto beads. These κaAPCs deplete targeted antigen-specific T cells in a Fas/Fas ligand (FasL)-dependent fashion. T-cell depletion in cocultures is rapidly initiated (30 minutes), dependent on the amount of κaAPCs and independent of activation-induced cell death (AICD). κaAPCs represent a novel technology that can control T cell-mediated immune responses, and therefore has potential for use in treatment of autoimmune diseases and allograft rejection.

Original languageEnglish (US)
Pages (from-to)3546-3552
Number of pages7
JournalBlood
Volume111
Issue number7
DOIs
StatePublished - Apr 1 2008

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Schütz, C., Fleck, M., Mackensen, A., Zoso, A., Halbritter, D., Schneck, J. P., & Oelke, M. (2008). Killer artificial antigen-presenting cells: A novel strategy to delete specific T cells. Blood, 111(7), 3546-3552. https://doi.org/10.1182/blood-2007-09-113522