Kif5b controls the localization of myofibril components for their assembly and linkage to the myotendinous junctions

Zai Wang, Ju Cui, Wai Man Wong, Xiuling Li, Wenqian Xue, Raozhou Lin, Jing Wang, Peigang Wang, Julian A. Tanner, Kathryn S.E. Cheah, Wutian Wu, Jian Dong Huang

Research output: Contribution to journalArticlepeer-review

Abstract

Controlled delivery of myofibril components to the appropriate sites of assembly is crucial for myofibrillogenesis. Here, we show that kinesin-1 heavy chain Kif5b plays important roles in anterograde transport of α-sarcomeric actin, non-muscle myosin IIB, together with intermediate filament proteins desmin and nestin to the growing tips of the elongating myotubes. Mice with Kif5b conditionally knocked out in myogenic cells showed aggregation of actin filaments and intermediate filament proteins in the differentiating skeletal muscle cells, which further affected myofibril assembly and their linkage to the myotendinous junctions. The expression of Kif5b in mutant myotubes rescued the localization of the affected proteins. Functional mapping of Kif5b revealed a 64-amino acid α-helix domain in the tail region, which directly interacted with desmin and might be responsible for the transportation of these proteins in a complex.

Original languageEnglish (US)
Pages (from-to)617-626
Number of pages10
JournalDevelopment (Cambridge)
Volume140
Issue number3
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

Keywords

  • Desmin
  • Kif5b
  • Myofibrils
  • Myotendinous junctions
  • Nestin

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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