Key roles of hydrophobic rings of TM2 in gating of the α9α10 nicotinic cholinergic receptor

Paola V. Plazas, María J. De Rosa, María E. Gomez-Casati, Miguel Verbitsky, Noelia Weisstaub, Eleonora Katz, Cecilia Bouzat, Ana Belén Elgoyhen

Research output: Contribution to journalArticle

Abstract

We have performed a systematic mutagenesis of three hydrophobic rings (17′, 13′ and 9′) within transmembrane region (TM) 2 of the α9α10 nicotinic cholinergic receptor (nAChR) to a hydrophilic (threonine) residue and compared the properties of mutant receptors reconstituted in Xenopus laevis oocytes. Phenotypic changes in α9α10 mutant receptors were evidenced by a decrease in the desensitization rate, an increase in both the EC 50 for ACh as well as the efficacy of partial agonists and the reduction of the allosteric modulation by extracellular Ca 2+. Mutated receptors exhibited spontaneous openings and, at the single-channel level, an increased apparent mean open time with no major changes in channel conductance, thus suggesting an increase in gating of the channel as the underlying mechanism. Overall, the degrees of the phenotypes of mutant receptors were more overt in the case of the centrally located V13′T mutant. Based on the atomic model of the pore of the electric organ of the Torpedo ray, we can propose that the interactions of side chains at positions 13′ and 9′ are key ones in creating an energetic barrier to ion permeation. In spite of the fact that the roles of the TM2 residues are mostly conserved in the distant α9α10 member of the nAChR family, their mechanistic contributions to channel gating show significant differences when compared to other nAChRs. These differences might be originated from slight differential intramolecular rearrangements during gating for the different receptors and might lead each nAChR to be in tune with their physiological roles.

Original languageEnglish (US)
Pages (from-to)963-974
Number of pages12
JournalBritish Journal of Pharmacology
Volume145
Issue number7
DOIs
StatePublished - Aug 2005
Externally publishedYes

Fingerprint

Electric Organ
Torpedo
Xenopus laevis
Nicotinic Receptors
Cholinergic Receptors
Threonine
Mutagenesis
Oocytes
Ions
Phenotype
Psychologic Desensitization

Keywords

  • Acetylcholine
  • Channel gating
  • Cys-loop receptors
  • Ionotropic receptors
  • Nicotinic receptors

ASJC Scopus subject areas

  • Pharmacology

Cite this

Plazas, P. V., De Rosa, M. J., Gomez-Casati, M. E., Verbitsky, M., Weisstaub, N., Katz, E., ... Elgoyhen, A. B. (2005). Key roles of hydrophobic rings of TM2 in gating of the α9α10 nicotinic cholinergic receptor. British Journal of Pharmacology, 145(7), 963-974. https://doi.org/10.1038/sj.bjp.0706224

Key roles of hydrophobic rings of TM2 in gating of the α9α10 nicotinic cholinergic receptor. / Plazas, Paola V.; De Rosa, María J.; Gomez-Casati, María E.; Verbitsky, Miguel; Weisstaub, Noelia; Katz, Eleonora; Bouzat, Cecilia; Elgoyhen, Ana Belén.

In: British Journal of Pharmacology, Vol. 145, No. 7, 08.2005, p. 963-974.

Research output: Contribution to journalArticle

Plazas, PV, De Rosa, MJ, Gomez-Casati, ME, Verbitsky, M, Weisstaub, N, Katz, E, Bouzat, C & Elgoyhen, AB 2005, 'Key roles of hydrophobic rings of TM2 in gating of the α9α10 nicotinic cholinergic receptor', British Journal of Pharmacology, vol. 145, no. 7, pp. 963-974. https://doi.org/10.1038/sj.bjp.0706224
Plazas PV, De Rosa MJ, Gomez-Casati ME, Verbitsky M, Weisstaub N, Katz E et al. Key roles of hydrophobic rings of TM2 in gating of the α9α10 nicotinic cholinergic receptor. British Journal of Pharmacology. 2005 Aug;145(7):963-974. https://doi.org/10.1038/sj.bjp.0706224
Plazas, Paola V. ; De Rosa, María J. ; Gomez-Casati, María E. ; Verbitsky, Miguel ; Weisstaub, Noelia ; Katz, Eleonora ; Bouzat, Cecilia ; Elgoyhen, Ana Belén. / Key roles of hydrophobic rings of TM2 in gating of the α9α10 nicotinic cholinergic receptor. In: British Journal of Pharmacology. 2005 ; Vol. 145, No. 7. pp. 963-974.
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