Ketogenic diet: Effects on expression of kindled seizures and behavior in adult rats

Ariyuki Hori, Pushpa Tandon, Gregory L. Holmes, Carl E. Stafstrom

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: Despite use of the ketogenic diet (KD) for >75 years its effectiveness or mechanism of action has been examined in few animal studies. Using the kindling model of epilepsy, we tested the anticonvulsant effectiveness and behavioral consequences of an experimental KD in adult rats. Methods: Rats fully kindled from the amygdala were divided into KD-fed or standard rat diet-fed groups; diet treatment continued for 5 weeks. The KD approximated at 4:1 ('classic') ketogenic diet and consisted (by weight) of 70% fat. 14% protein, no carbohydrate, and appropriate vitamins, minerals and fiber; 92% of energy provided was contributed by fat and 8% was contributed by protein. Afterdischarge threshold and duration (ADT, ADD) and stage 5 seizure threshold and duration (ST, SD) were assessed weekly for 5 weeks. During week 3, learning and memory were tested by the water maze and the behavioral response to a novel environment was assessed by the open field test. Results: Rats receiving the KD became ketonemic and had weight gains similar to those of control rats. As compared with rats receiving a standard diet, those fed the KD had an elevated ADT and ST for the first 2 weeks of treatment. The control and KD-fed groups did not differ with regard to ADD or SD at any time during the study, and both groups performed similarly in the water maze and open field test. Conclusions: In the kindling model, the KD afforded transient protection against the focal generation of kindled seizures but not seizure spread. Rats that received the KD did not perform differently from control-fed rats on spatial learning or exploratory behavior tasks. Our results provide a promising model for study of the anticonvulsant mechanisms of ketosis.

Original languageEnglish (US)
Pages (from-to)750-758
Number of pages9
JournalEpilepsia
Volume38
Issue number7
DOIs
StatePublished - 1997

Keywords

  • Afterdischarge threshold
  • Ketogenic diet
  • Kindling
  • Seizure
  • Water maze

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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