Keratinocyte urokinase-type plasminogen activator is secreted as a single chain precursor

Koji Hashimoto, Janet H. Prystowsky, Janet Baird, Gerald S. Lazarus, Pamela J. Jensen

Research output: Contribution to journalArticle

Abstract

Urokinase-type plasminogen activator (uPA) is produced and secreted by cultured human keratinocytes as a single chain precursor. UPA in keratinocyte conditioned medium is not susceptible to inhibition with diisopropylfluorophosphate (DFP), and it has an apparent molecular weight of 55 kD under both reducing and nonreducing conditions. Cleavage of keratinocyte uPA by plasmin results in the formation of a 96 kD complex comprised of activated uPA and PA inhibitor 2. PA extracted from normal human epidermis is only partially inhibited by DFP, suggesting that precursor uPA is also present in vivo. The synthesis of uPA as a precursor with reduced enzymatic activity as well as decreased affinity for inhibitors is likely to be a mechanism by which normal epidermis regulates plasminogen activation in vivo.

Original languageEnglish (US)
Pages (from-to)823-828
Number of pages6
JournalJournal of Investigative Dermatology
Volume90
Issue number6
DOIs
StatePublished - Jun 1988
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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