Keratectasia after laser in situ keratomileusis: A histopathologic and immunohistochemical study

Beeran Meghpara, Hiroshi Nakamura, Marian Macsai, Joel Sugar, Ahmed Hidayat, Beatrice Y J T Yue, Deepak P. Edward

Research output: Contribution to journalArticle

Abstract

Objective: To examine histopathologic and immunohistochemical features of human corneal buttons from patients who developed keratectasia after laser in situ keratomileusis (LASIK). Methods: Five corneal buttons were obtained during penetrating keratoplasty from patients who developed keratectasia after LASIK. Histologic features were examined by hematoxylin-eosin staining using paraffinembedded sections and by transmission electron microscopy. Immunostaining for α1-proteinase inhibitor, Sp1, and matrix metalloproteinases 1, 2, and 3 was performed with 2 healthy corneas and 2 corneas with keratoconus as controls. Results: Central stromal thinning was observed after hematoxylin-eosin staining in all corneas with keratectasia. No histologic features specific to keratoconus, including Bowman layer disruption, were identified in the corneas with keratectasia. By transmission electron microscopy, collagen fibril thinning and decreased interfibril distance were observed in the stromal bed. Immunostaining intensity and/or pattern for α1-proteinase inhibitor and Sp1 in the corneas with keratectasia was comparable to that of healthy corneas and differed from that in the corneas with keratoconus. No significant staining with anti-matrix metalloproteinases 1, 2, and 3 antibodies was observed in either the corneas with keratectasia or the healthy corneas. Conclusions: Histologic findings suggest that post-LASIK keratectasia results in collagen fibril thinning and decreased interfibril distance within the residual stromal bed. Discrepant results between keratectasia and keratoconus suggest that the pathogenesis of the 2 conditions differ.

Original languageEnglish (US)
Pages (from-to)1655-1663
Number of pages9
JournalArchives of Ophthalmology
Volume126
Issue number12
DOIs
StatePublished - Dec 2008
Externally publishedYes

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Laser In Situ Keratomileusis
Cornea
Keratoconus
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Matrix Metalloproteinase 2
Hematoxylin
Eosine Yellowish-(YS)
Staining and Labeling
Transmission Electron Microscopy
Peptide Hydrolases
Collagen
Penetrating Keratoplasty
Antibodies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Meghpara, B., Nakamura, H., Macsai, M., Sugar, J., Hidayat, A., Yue, B. Y. J. T., & Edward, D. P. (2008). Keratectasia after laser in situ keratomileusis: A histopathologic and immunohistochemical study. Archives of Ophthalmology, 126(12), 1655-1663. https://doi.org/10.1001/archophthalmol.2008.544

Keratectasia after laser in situ keratomileusis : A histopathologic and immunohistochemical study. / Meghpara, Beeran; Nakamura, Hiroshi; Macsai, Marian; Sugar, Joel; Hidayat, Ahmed; Yue, Beatrice Y J T; Edward, Deepak P.

In: Archives of Ophthalmology, Vol. 126, No. 12, 12.2008, p. 1655-1663.

Research output: Contribution to journalArticle

Meghpara, B, Nakamura, H, Macsai, M, Sugar, J, Hidayat, A, Yue, BYJT & Edward, DP 2008, 'Keratectasia after laser in situ keratomileusis: A histopathologic and immunohistochemical study', Archives of Ophthalmology, vol. 126, no. 12, pp. 1655-1663. https://doi.org/10.1001/archophthalmol.2008.544
Meghpara, Beeran ; Nakamura, Hiroshi ; Macsai, Marian ; Sugar, Joel ; Hidayat, Ahmed ; Yue, Beatrice Y J T ; Edward, Deepak P. / Keratectasia after laser in situ keratomileusis : A histopathologic and immunohistochemical study. In: Archives of Ophthalmology. 2008 ; Vol. 126, No. 12. pp. 1655-1663.
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