Juvenile human RPE graft rescues photoreceptor cells in dystrophic RCS rats

M. Del Cerro, B. V. Castillo, R. White, Christopher Cox, J. Wyatt, G. Nadiga, C. Del Cerro

Research output: Contribution to journalArticle

Abstract

Purpose: To investigate the utility of non-fetal human RPE for subretinal transplantation in dystrophic RCS rats. Methods: Two eyes from a 10 year old female donor were obtained through the Rochester Eye and Human Parts Bank. The eyes were held in Optisol for less than 24 hrs. The RPE was isolated by enzymatic treatment of the choroid-RPE with 2% dispase for 30 min. at 37°C (Castillo et al. 1995 Curr. Eye Res 1:677). Mechanically dissociated RPE was injected into the subretinal space of P21 dystrophic RCS rats at the superior equatorial (SE) region using the transcleral technique of del Cerro et al. (1992 J. Neurosci. Meth. 43:157). Rats receiving vehicle injection served as sham controls. The animals were immunosuppresed with daily cyclosporine injections (10 mg/kg). At 4 wks post-transplantation, the fundi were examined and the eyes were enucleated for histologic evaluation and morphometric analysis of PRC survival. Results: Fundoscopic examination revealed survival of the juvenile RPE graft which varied from large dense focal collections to small scattered islands of pigmented cells. Histologically, the grafted eyes exhibited greater number of surviving photoreceptor cells. Morphometric analysis of 1 μm thick sections demonstrated a 6 fold increase in PRC density at the SE region of grafted eyes (62.10 ± 29.3) compared to the same region of sham controls (10.75 ± 2.2) (p

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

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Photoreceptor Cells
Transplants
Transplantation
Injections
Choroid
Survival Analysis
Cyclosporine
Survival

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Del Cerro, M., Castillo, B. V., White, R., Cox, C., Wyatt, J., Nadiga, G., & Del Cerro, C. (1996). Juvenile human RPE graft rescues photoreceptor cells in dystrophic RCS rats. Investigative Ophthalmology and Visual Science, 37(3).

Juvenile human RPE graft rescues photoreceptor cells in dystrophic RCS rats. / Del Cerro, M.; Castillo, B. V.; White, R.; Cox, Christopher; Wyatt, J.; Nadiga, G.; Del Cerro, C.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Del Cerro, M, Castillo, BV, White, R, Cox, C, Wyatt, J, Nadiga, G & Del Cerro, C 1996, 'Juvenile human RPE graft rescues photoreceptor cells in dystrophic RCS rats', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Del Cerro, M. ; Castillo, B. V. ; White, R. ; Cox, Christopher ; Wyatt, J. ; Nadiga, G. ; Del Cerro, C. / Juvenile human RPE graft rescues photoreceptor cells in dystrophic RCS rats. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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