IspG-Catalyzed Positional Isotopic Exchange in Methylerythritol Cyclodiphosphate of the Deoxyxylulose Phosphate Pathway: Mechanistic Implications

Youli Xiao; Debra Rooker; Quincy You; Caren L. Freel Meyers; Pinghua Liu

doi:10.1002/cbic.201000716

IspG-Catalyzed Positional Isotopic Exchange in Methylerythritol Cyclodiphosphate of the Deoxyxylulose Phosphate Pathway: Mechanistic Implications

Youli Xiao, Debra Rooker, Quincy You, Caren L. Freel Meyers, Pinghua Liu

School of Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

H ₂ ¹⁸O under the bridge: Recently, the deoxyxylulose phosphate (DXP) pathway was discovered to be a second pathway supplying isoprenoid biosynthetic precursors. One of steps is an IspG-catalyzed reductive deoxygenation of methylerythritol cyclodiphosphate (MEcPP) to 4-hydroxyl-3-methyl-2-(E)-1-diphosphate (HMBPP). Using [2- ¹³C, ¹⁸O]-MEcPP, we detected the positional isotopic exchange for the bridging oxygen in MEcPP.

Original language	English (US)
Pages (from-to)	527-530
Number of pages	4
Journal	ChemBioChem
Volume	12
Issue number	4
DOIs	https://doi.org/10.1002/cbic.201000716
State	Published - Mar 7 2011

Keywords

Biosynthesis
DXP pathway
Isoprenoids
IspG
Positional isotopic exchange

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.201000716

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title = "IspG-Catalyzed Positional Isotopic Exchange in Methylerythritol Cyclodiphosphate of the Deoxyxylulose Phosphate Pathway: Mechanistic Implications",

abstract = "H 2 18O under the bridge: Recently, the deoxyxylulose phosphate (DXP) pathway was discovered to be a second pathway supplying isoprenoid biosynthetic precursors. One of steps is an IspG-catalyzed reductive deoxygenation of methylerythritol cyclodiphosphate (MEcPP) to 4-hydroxyl-3-methyl-2-(E)-1-diphosphate (HMBPP). Using [2- 13C, 18O]-MEcPP, we detected the positional isotopic exchange for the bridging oxygen in MEcPP.",

keywords = "Biosynthesis, DXP pathway, Isoprenoids, IspG, Positional isotopic exchange",

author = "Youli Xiao and Debra Rooker and Quincy You and {Freel Meyers}, {Caren L.} and Pinghua Liu",

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T1 - IspG-Catalyzed Positional Isotopic Exchange in Methylerythritol Cyclodiphosphate of the Deoxyxylulose Phosphate Pathway

T2 - Mechanistic Implications

AU - Xiao, Youli

AU - Rooker, Debra

AU - You, Quincy

AU - Freel Meyers, Caren L.

AU - Liu, Pinghua

PY - 2011/3/7

Y1 - 2011/3/7

N2 - H 2 18O under the bridge: Recently, the deoxyxylulose phosphate (DXP) pathway was discovered to be a second pathway supplying isoprenoid biosynthetic precursors. One of steps is an IspG-catalyzed reductive deoxygenation of methylerythritol cyclodiphosphate (MEcPP) to 4-hydroxyl-3-methyl-2-(E)-1-diphosphate (HMBPP). Using [2- 13C, 18O]-MEcPP, we detected the positional isotopic exchange for the bridging oxygen in MEcPP.

AB - H 2 18O under the bridge: Recently, the deoxyxylulose phosphate (DXP) pathway was discovered to be a second pathway supplying isoprenoid biosynthetic precursors. One of steps is an IspG-catalyzed reductive deoxygenation of methylerythritol cyclodiphosphate (MEcPP) to 4-hydroxyl-3-methyl-2-(E)-1-diphosphate (HMBPP). Using [2- 13C, 18O]-MEcPP, we detected the positional isotopic exchange for the bridging oxygen in MEcPP.

KW - Biosynthesis

KW - DXP pathway

KW - Isoprenoids

KW - IspG

KW - Positional isotopic exchange

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IspG-Catalyzed Positional Isotopic Exchange in Methylerythritol Cyclodiphosphate of the Deoxyxylulose Phosphate Pathway: Mechanistic Implications

Abstract

Keywords

ASJC Scopus subject areas

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