Previously, the specificity of human immune responses to Dactylis pollen was analyzed in 26 plasma samples with high levels of grass-pollen-specific lgG4 (‘IgG4+ plasma’, largely from grass-pollen-allergic patients), as compared to 25 plasma samples with low grass-pollen-specific IgG4 (‘normal plasma’, from nonatopic individuals). In the present study, a quantification of the Dactylis- pollen-spccific IgE, IgM, IgA class and IgG subclass antibodies in these plasma samples is proposed. Isotypic distribution in IgG4+ plasma was 68% IgG [IgG2 (38%) > IgG4 (30%) > IgG 1 (19%) > IgG3 (13%)], 27% IgM, 4% IgA and 0.05% IgE, In normal plasma it was 73% IgM, 20% IgG [IgG3 (38%) > IgG2 (33%) > IgG 1 (29%) > IgG4 (()%)]. 6% IgA and 0.006% IgE, In IgG4+ plasma, specific IgE, IgG I, IgG2 and IgG4 concentrations were positively correlated between each other. Finally, the present study clearly confirmed the possible role of the CH gene regulation in allergic diseases.
- Dactylis glomerate (cocksfoot)
- Grass pollen
- Human humoral immune response
- IgG 1-4
ASJC Scopus subject areas
- Immunology and Allergy