Isolation of YAC insert sequences by representational difference analysis

Mieke Schutte, Luis T. Da Costa, Christopher A. Moskaluk, Ester Rozenblum, Xiaoping Guan, Pieter J. De Jong, Michael Bittner, Paul S. Meltzer, Jeffrey M. Trent, Scott E. Kern

Research output: Contribution to journalArticle

Abstract

We present a method for the isolation of YAC insert sequences by representational difference analysis (RDA). To achieve maximal representation of the sequences, the amplicons were generated from a Mbol digestion product. RDA was performed using a 970 kb insert YAC clone. After two rounds of re-association and selective amplification 92% of the difference product represented sequences derived from the YAC insert. Twenty insert-specific sequence-tagged sites were readily defined. The difference product was also successfully used to isolate microsatellite markers, to identify clones from a human PAC library and as a chromosome painting probe in fluorescence in situ hybridization.

Original languageEnglish (US)
Pages (from-to)4127-4133
Number of pages7
JournalNucleic acids research
Volume23
Issue number20
DOIs
StatePublished - Oct 25 1995

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Isolation of YAC insert sequences by representational difference analysis'. Together they form a unique fingerprint.

  • Cite this

    Schutte, M., Da Costa, L. T., Moskaluk, C. A., Rozenblum, E., Guan, X., De Jong, P. J., Bittner, M., Meltzer, P. S., Trent, J. M., & Kern, S. E. (1995). Isolation of YAC insert sequences by representational difference analysis. Nucleic acids research, 23(20), 4127-4133. https://doi.org/10.1093/nar/23.20.4127