Isolation of the murine branched-chain α-ketoacid dehydrogenase E2 subunit promoter region

Paul A. Costeas, Jeffrey M. Chinsky

Research output: Contribution to journalArticle

Abstract

The promoter region of the murine branched-chain α-ketoacid dehydrogenase E2 subunit (dihydrolipoyl transacylase) gene was isolated and characterized. Sequence analysis of the promoter-regulatory region showed the presence of two inverted 'CAAT box' sequences, the most proximal being -42 to -48 bp upstream from the determined transcription initiation site, but no TATA-box sequences, similar to the human BCKAD E2 gene. The boundary of the minimum promoter sequence appeared to reside just inclusive of this first inverted CAAT sequence, but minigene transfer analysis demonstrated that the promoter proximal region between -65 and -140 bp is likely to be extremely important for controlling regulated changes in E2 RNA expression. The regulatory effect of this region may be modulated by a number of other upstream regions which were identified within the 7.0 kb sequence examined.

Original languageEnglish (US)
Pages (from-to)111-116
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1399
Issue number1
DOIs
StatePublished - Jul 30 1998

Keywords

  • Branched-chain α-ketoacid dehydrogenase
  • Dihydrolipoyl transacylase
  • E2 subunit
  • Promoter

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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