Isolation of rsp-1, a Novel cDNA Capable of Suppressing v-Ras Transformation

M. L. Cutler, R. H. Bassin, L. Zanoni, N. Talbot

Research output: Contribution to journalArticle

Abstract

Using an expression cloning assay, we have isolated a novel cDNA, referred to as rsp-1, which suppresses the v-Ras-transformed phenotype. When introduced into NIH 3T3 fibroblasts under the control of a metallothionein promoter, rsp-1 confers resistance to v-Ras, but not to v-Mos or v-Src, and inhibits growth of the cells. The rsp-1 cDNA contains a 831-bp open reading frame encoding a 277-amino-acid leucine-rich protein. The rsp-1 cDNA exhibits no significant homology to sequences in the DNA data bases. However, searches of the protein data bases revealed that it contains a series of leucine-based repeats which are homologous to the leucine repeats found in the regulatory region of the yeast adenylyl cyclase. rsp-1 specific RNA is detectable in a wide variety of cell lines and tissues, and the gene is conserved among eukaryotic species. These data suggest that rsp-1 plays a role in Ras signal transduction.

Original languageEnglish (US)
Pages (from-to)3750-3756
Number of pages7
JournalMolecular and cellular biology
Volume12
Issue number9
DOIs
StatePublished - Sep 1992

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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